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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
Ulum, M. F.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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Journal : Tropical Animal Science Journal

 1 
A Novel SNPs of the SREBF1 and SCARB1 Genes and the Association with Fatty Acid Profile in Bali Cattle Dairoh; Ulum, M. F.; Jakaria; Ishak, A. B. L.; Sumantri, C.
Tropical Animal Science Journal Vol. 46 No. 4 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.4.428

Abstract

This study aimed to investigate the genetic impact of single nucleotide polymorphisms (SNPs) of the sterol regulating element binding factor 1 (SREBF1) and scavenger receptor class B member 1 (SCARB1) genes on carcass and meat characteristics, as well as fatty acid composition, in the Bali cattle. The blood and beef samples used for DNA sequencing, physical assessment, and fatty acid analysis were collected from 95 male Bali cattle. The ultrasound images were analyzed using the Image-J NIH software. A total of 4 SNPs were identified in the SREBF1 gene and 5 SNPs in the SCARB1 gene. The results showed that the 4 SNPs in the SREBF1 gene, namely g.12629T>C, g.12731T>C, g.12881A>G, and g.12986C>T, were associated with heptadecanoic acid (C17:0) and cis-11-eicosanoic acid (C20:1). The SNPs g.12731T>C of the SREBF1 gene was associated with fat content, palmitoleic acid (C16:1), stearic acid (C18:0), cis-11-eicosanoic acid (C20:1), and total fatty acids. Furthermore, 4 SNPs in the SCARB1 gene, including g.72219C>T, g.72380C>A, g.72517G>A, and g.72607C>T correlated with longissimus dorsi thickness (LDT). All SNPs in the SCARB1 gene showed significant associations with cis-10 heptadecanoic acid (C17:1) and cis 8,11,14-eicosatrienoic acid (C20:3n6). The SNP g.72400A>G of the SCARB1 gene was related to caprylic acid (C8:0), lauric acid (C12:0), arachidonic acid (C20:4n6), monounsaturated fatty acids (MUFA), and unsaturated fatty acids (UFA). These results suggested that the identified polymorphisms in the SREBF1 and SCARB1 genes could serve as valuable references for investigating similar genes in other cattle breeds, particularly concerning fatty acids.
SNP Detection in FREM2 Gene and Its Association with Carcass Quality in Bali Beef Pertiwi, E. A.; Ulum, M. F.; Jakaria, J.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.149

Abstract

The FRAS1-related extracellular matrix protein 2 (FREM2) gene is one of the genes that play a role in controlling marbling scores in beef cattle. This study aimed to identify SNPs in exon 6 of the FREM2 gene and its association with carcass quality in Bali beef using ultrasonography. A total of 93 cattle were used: 55 cattle from Banjarmasin slaughterhouse, South Kalimantan, Indonesia, 28 cattle from Bali Cattle Breeding Centre in Bali Province, and 10 cattle from UPTD Kupang Regency, NTT, Indonesia. SNP of the FREM2 gene was identified by using sequencing techniques and then genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The association of FREM2 gene SNPs with carcass characteristics was analyzed using the General Linear Model (GLM) method using SPSS software version 22. Carcass (longissimus dorsi thickness; back fat thickness) and meat (intramuscular fat; marbling score) characteristics were identified non-invasively using ultrasonography images and then analyzed with Image-J NIH software. SNP at position g.89327G>A was located in exon 6 of the FREM2 gene and did not change the amino acid (prolineproline) sequence in Bali beef. The genotyping results with PCR-RFLP technique SNP g.89327G>A FREM2|BccI gene has high diversity. The alleles of A and G were 0.747 and 0.253, respectively. The diversity of SNP g.89327G>A was significantly associated (p<0.05) with longissimus dorsi thickness (LD), while back fat thickness, intramuscular fat, and marbling score did not significantly different (p>0.05). Thus, SNP at position g.89327G>A in exon 6 of the FREM2 gene might be used as a candidate genetic marker for carcass quality in Bali beef.
Co-Authors A. B. L. Ishak, A. B. L. C. SUMANTRI Dairoh Dairoh, D. Jakaria Jakaria Khasanah, H. Kholijah, K. Londra, I. M. M. Baihaqi Pertiwi, E. A. R. Priyanto R. R. Noor, R. R. S. Darwati Saputra, E. A.