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Sobir, dan

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Author-ID : 1119920

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Keragaman Genetik Bawang Merah (Allium cepa L.) Berdasarkan Marka Morfologi dan ISSR Sari, Vebrita; ,, Miftahudin; Sobir, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Development of new shallot varieties requires genetic variation of the germplasm. However, information on genetic diversity of local shallot in Indonesia is still lacking. This study was aimed to analyze the genetic diversity of shallot based on morphological characters and ISSR markers for management of genetic resource and breeding program in Indonesia. This study was conducted during October 2014 to September 2015. The 34-shallot genotypes were collected from several regions in Indonesia in the form of the bulb. Twenty four morphological markers and 13 ISSR primers were used in this research. The informative characters to analyze genetic diversity of shallot were foliage cracking, bulb number and bulb morphological characters. ISSR markers generated 103 DNA polymorphic band with the total of 89.57% and the informative primers were ISSRred 4, ISSRred 9 and ISSRred 20. Both morphological and ISSR markers showed 27% genetic diversity and grouped all genotypes into two main groups; however the grouping did not relate to the geographic origin. The largest bulb diameter of 7.54 cm was obtained from Bangkok variety and the highest number of bulb per clump of 27 bulb/clump was produced by the genotype from Pekanbaru. These genotypes can be used as potential parents for shallot breeding program in Indonesia. Keywords: genetic diversity, ISSR markers, morphological characters, shallot

Pewarisan Sifat Karakter Kualitatif dan Kuantitatif pada Hipokotil dan Kotiledon Cabai (Capsicum annuum L.) Rtionga, Arya Widura; Syukur, Muhamad; Yunianti, Rahmi; Sobir, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Characters on hypocotyl dan cotyledon are very potential used for effective and efficient morphology marker for some crop plants. However, the information about the inheritance of qualitative and quantitative characters on hypocotyl and cotyledon of chili pepper was not available. The aim of this research was to determine the inheritance of qualitative and quantitative characters on hypocotyl and cotyledon of chili pepper. This research used purple chili pepper (P1 (IPB C20)), green chili pepper (P2 (IPB C2)), F1, F1R, BCP1, BCP2, and F2 populations. Analysis for qualitative characters used mendelian genetics analysis, whereas model genetic prediction for quantitative characters used join scaling test analysis. The result showed that hypocotyl and cotyledon color of chili pepper controlled by single gene. The gene controlling purple color was dominant than the gene controlling green color on hypocotyl, whereas the gene controlling green color was dominant than the gene controlling purple color on cotyledon. The additive-dominant genetic model with influence of additive-additive and additive-dominant interaction was suitable for hypocotyl length. The additive-dominant genetic model with influence of additive-dominant and dominant-dominant interaction was suitable for hypocotyls diameter. The additive-dominant genetic model with influence of additive-additive and dominant-dominant interaction was suitable for cotyledon width and cotyledon length. Keywords: genetic model, morfology marker, natural cross-pollination

Induksi Mutasi Tanaman Leunca (Solanum nigrum L.) untuk Meningkatkan Keragaman Kandungan Tanin Saragih, Siti Hartati Yusida; Aisyah, Syarifah Iis; Sobir, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Leunca (Solanum nigrum L. ) is one of the indigenous vegetables in Indonesia. The fruit of this vegetable is green and has bitter taste because of its tannin content. The objectives of this study were to get lethal dose 50 (LD50), diversity and to obtain candidates of mutant plants with high and low tannin content in young fruit and ripe fruit in leunca. The research was conducted at Pasir Kuda experimental station, Bogor; National Nuclear Energy Agency (BATAN), Jakarta; and postharvest laboratory of the Center for Tropical Horticulture Studies (PKHT IPB), Bogor, from December 2016 to July 2017. Leunca accession of SN 20 and SN 29 were irradiated using doses of 0, 100, 150, 200, and 250 Gy of gamma ray. The LD50 values for SN 20 and SN 29 were 171.944 Gy and 190.949 Gy, respectively. The analysis of tannin content was performed based on permanganate method by titration using potassium permanganate. The results showed that the highest tannin content of green stage fruit was found in SN20D3.5 with 200 Gy dose and the lowest was found in SN29D1.5 with 100 Gy dose. The highest tannin content of ripe fruit was found in SN20D4.6 with dose of 250 Gy and the lowest was found in SN20D3.1 with dose of 200 Gy. Recommended tannin content for leunca is the low tannin content.Keywords: diversity, gamma ray, indigenous vegetable, irradiation, LD50

Pewarisan Karakter Hasil dan Komponen Hasil pada Dua Populasi Persilangan Cabai Rawit Hijau (Capsicum annuum L.) Yudilastari, Tiara; Syukur, Muhamad; Sobir, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 3 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Genetic information plays an important role in plant selection. This research was aimed to obtain genetic information on pattern inheritance of yield and yield component characters of two crosses population of birds chili. The research used biparental mating design consisted of 40 plants of parents population (P1 and P2), 40 plants of first generation (F1) and reciprocal (F1R) populations, 100 plants of backcross populations (BCP1 and BCP2), and 300 plants of second generation population (F2). Scaling test and joint scaling test indicated the presence of gene interaction (epistasis) in two crosses population. Estimates gene effect also showed that fruit weight character in crosses population of IPB C145 x IPB C174 was controlled by duplication epistasis, while yield per plant character in crosses population of IPB C145 x IPB C291 was controlled by complementary epistasis. All characters of both crosses populations had high broad sense heritability, but the characters in crosses population of IPB C145 x IPB C174 had medium to high narrow sense heritability, while the characters in crosses population of IPB C145 x IPB C291 had low to medium narrow sense heritability. Genetic difference between parents seemed to affect gene action of a character, in which yield per plant, number of fruits per plant, fruit weight, and fruit length characters in crosses population of IPB C145 x IPB C291 with small genetic difference were more controlled by the action of non additive gene than crosses population of IPB C145 x IPB C291 with large genetic difference.Keywords: epistasis, gene action, genetic difference, heritability, yield

Co-Authors Miftahudin ,, Miftahudin Muhamad Syukur RAHMI YUNIANTI Rtionga, Arya Widura Sari, Vebrita Siti Hartati Yusida Saragih Syarifah Iis Aisyah Yudilastari, Tiara

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