Introduction: Chemotherapy is one of the cancer treatments, which often causes disturbing side effects. Educational knowledge about chemotherapy can reduce anxiety and increase patient knowledge. Digital-based health applications, such as CheMonD, show great potential in improving the quality of cancer patients during chemotherapy. This study aims to determine the effect of chemotherapy education through digital supportive services in the form of application CheMonD to knowledge patient which will undergo chemotherapy and assess the satisfaction of users of these services.Method: research is a usability testing with a quasi-experiment using a post-test design conducted in the Nakula 5 One Day Care Room: Chemotherapy RSA UGM , Yogyakarta. Inclusion criteria: patients which get chemotherapy first, age ≥ 18 year, score Karnofsky > 50, able to communicate effectively and willing to be involved in the study. Exclusion criteria were: patients did not have gadgets, patients with cognitive disorders based on medical record data. Respondents received chemotherapy education through an Android-based digital application containing videos, educational narratives and supportive services. At the end, a knowledge and satisfaction questionnaire was given user application. Analysis data use analysis univariate.Results: A total of 22 respondents participated in the usability testing. From the knowledge aspect, the average correct answer of respondents was 9.45 (52.52%). Most respondents still did not understand as indicated by the majority of wrong answers on aspects of questions about preventing canker sores, preventing constipation, preventing bleeding, and overcoming baldness. The satisfaction value was at 80.06% with details of each domain of satisfaction with educational content 79.82%, accuracy level 84.09%, application format 80.3%, ease of use 79.54% and time effectiveness 76.70%.Conclusion: Patient knowledge related to the prevention of mouth ulcers, constipation, bleeding and baldness is an aspect of knowledge that is still low. Respondent satisfaction with the application is 80.06%. The educational aspect related to symptom prevention can be improved in the preparation of the application while still considering the domain of convenience and time effectiveness.