This study aims to analyze the characteristics and needs of a biodiversity e-module integrated with local wisdom to support critical thinking and collaboration skills among junior high school students in Sorong Regency. The study employed a descriptive qualitative design using a Systematic Literature Review (SLR) strategy guided by PRISMA 2020, complemented by an analysis of local-context instructional documents. The research objects consisted of four teaching modules/lesson plans (RPP) on biodiversity and ecosystems from schools in Sorong Regency and surrounding areas, as well as scientific articles published between 2021 and 2026. The research procedures included literature identification and selection, analysis of local instructional documents, data extraction, categorization, thematic synthesis, and mapping of findings through a literature map. Data were collected by searching Google Scholar, GARUDA, and DOAJ using keywords related to e-modules, biodiversity, local wisdom, critical thinking, and collaboration. Data were analyzed through reduction, comparison, categorization, and thematic synthesis. The results showed that the four local instructional documents had implemented Project-Based Learning, Discovery Learning, and Problem-Based Learning; however, they had not explicitly integrated local wisdom or provided separate and measurable instruments for assessing critical thinking and collaboration. The literature synthesis indicated that e-modules integrating local wisdom, PBL, inquiry-based learning, and collaborative problem solving have potential to support both competencies. Therefore, the e-module should be contextual, interactive, problem- or inquiry-based, collaborative, and equipped with explicit assessment instruments.