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Trisna Agung Phabiola
Universitas Udayana, Indonesia
Arts Humanities Economics, Econometrics & Finance Education Law, Crime, Criminology & Criminal Justice Social Sciences

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Effectiveness of Lemongrass Against Fusarium oxysporum Wilt Disease in Tomato Alysa Clara Novi Ayu Sipayung; I Putu Sudiarta; Trisna Agung Phabiola
Research Horizon Vol. 6 No. 4 (2026): Research Horizon - Agustus 2026
Publisher : LifeSciFi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54518/rh.6.4.2026.1497

Abstract

Tomato (Solanum lycopersicum) production is severely constrained by Fusarium wilt caused by Fusarium oxysporum, a soil-borne pathogen that disrupts vascular tissues and reduces plant growth and productivity. Although synthetic fungicides are widely used, their environmental impacts have encouraged the development of sustainable alternatives. This study aimed to evaluate the effectiveness of citronella (Cymbopogon citratus) essential oil as a botanical pesticide for suppressing Fusarium wilt and improving tomato plant growth under greenhouse conditions. The experiment employed a Randomized Block Design consisting of five treatments (0, 0.25, 0.50, 0.75, and 1.00% Lemongrass) with five replications. Disease incidence and plant height were measured, and the data were analyzed using analysis of variance followed by Duncan’s Multiple Range Test at the 5% significance level. The results showed that Lemongrass significantly reduced disease incidence while enhancing plant growth. The 1.00% treatment produced the lowest disease incidence (10.00%) and the greatest plant height (25.00 cm), whereas the control treatment exhibited 100.00% disease incidence and the lowest plant height (7.00 cm). These findings indicate that citronella essential oil is a promising environmentally friendly botanical pesticide for managing Fusarium wilt while supporting tomato growth.
Antibacterial Activity of Leaf, Stem and Flower Extracts of Songgolangit Plant Against Staphylococcus aureus, Escherichia coli, and Pectobacterium sp. Yuliatin Rahman; I Ketut Suada; I Nyoman Wijaya; I Gede Putu Wirawan; Trisna Agung Phabiola; Khamdan Khalimi
Research Horizon Vol. 6 No. 4 (2026): Research Horizon - Agustus 2026
Publisher : LifeSciFi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54518/rh.6.4.2026.1505

Abstract

The increasing prevalence of antibiotic-resistant bacteria has encouraged the exploration of natural sources of antibacterial compounds, including Tridax procumbens L. This study aimed to evaluate the antibacterial activity of leaf, stem, and flower extracts of T. procumbens against Staphylococcus aureus, Escherichia coli, and Pectobacterium sp. Plant samples were collected in Denpasar, Bali, dried, powdered, and extracted by maceration using 96% ethanol, n-hexane, and ethyl acetate. Antibacterial activity was assessed using the disc diffusion method at extract concentrations of 10–50%, with 1% DMSO as the negative control. Inhibition zone diameters were analyzed using analysis of variance followed by the least significant difference test at the 5% significance level. Leaf and stem extracts showed no antibacterial activity against any tested bacteria. Antibacterial activity was observed only in the flower extracts against S. aureus, with the n-hexane extract producing the largest inhibition zone (10.0 mm at 50% concentration), followed by the ethyl acetate extract, while the ethanol extract was inactive. No extracts inhibited E. coli or Pectobacterium sp. These findings indicate that n-hexane flower extract possesses limited antibacterial activity against S. aureus, highlighting its potential as a source of antibacterial compounds and the need for further purification and characterization of its bioactive constituents.  
Co-Authors Alysa Clara Novi Ayu Sipayung I Gede Putu Wirawan I Ketut Suada I Nyoman Wijaya I Putu Sudiarta Khamdan Khalimi Yuliatin Rahman