<![CDATA[ Propolis is a natural ingredient containing various bioactive compounds, such as flavonoids, terpenoids, and phenolic compounds. Solid Lipid Nanoparticles (SLN) is one of the delivery systems that has the potential to overcome bioactive compounds that have limited bioavailability and solubility. This study aims to determine the effect of variations in Tween 80 concentration on the physical properties of SLN formulations of Trigona sp. propolis extract. Experimental research with SLN formulations using the heat homogenization method using variations in Tween 80 concentrations of 0.5%; 1%; 2%; and 3%. Evaluations were carried out on organoleptic, homogeneity, pH, viscosity, stability, percent transmittance (%T), and entrapment efficiency (%EP). All SLN formulas had good initial organoleptic characteristics and homogeneity. Better organoleptic stability in F3 (Tween 80 2%) and F4 (Tween 80 3%) due to minimizing sedimentation and phase separation. The pH value was in the weak acid range, while the optimum viscosity was obtained at F2 (Tween 80 1%). The %T values of F3 and F4 were 72.72% and 79.43%, respectively, indicating the formation of a nano-sized dispersion system. The overall %EP value was 73.05–77.94%, with the highest value at F2 (Tween 80 1%), indicating that most of the phenolic compounds of the propolis extract were successfully encapsulated in the SLN lipid matrix. Variations in Tween 80 concentration affected the physical properties of the SLN.]]>
