This study aims to examine the influence of the work environment on job stress among healthcare workers through a Systematic Literature Review (SLR) approach. High job demands in the healthcare sector place healthcare professionals at significant risk of experiencing job stress, which may negatively affect both individual well-being and the quality of healthcare services. This study utilizes secondary data obtained from national and international peer-reviewed journal articles published within the last ten years. The literature search was conducted systematically by applying predefined inclusion and exclusion criteria to ensure the relevance and quality of the selected studies. The data were analyzed using qualitative synthesis to identify patterns, similarities, differences, and research gaps across the reviewed literature. The findings indicate that the work environment, encompassing both physical and psychosocial dimensions, has a significant effect on job stress among healthcare workers. Key determinants include workload, organizational support, interpersonal relationships, leadership style, and working conditions. A supportive work environment is associated with lower levels of stress and burnout, whereas an inadequate environment contributes to increased emotional exhaustion and psychological strain. However, inconsistencies in several findings suggest the presence of additional moderating or mediating variables influencing this relationship. The study contributes to the theoretical development of human resource management in healthcare settings and provides practical implications for healthcare organizations in designing healthier and more productive work environments.