Anemia during pregnancy remains a public health problem in many countries, including Indonesia, especially among pregnant women in their third trimester who have increased physiological needs for iron and supporting nutrients. Maternal nutritional status is one of the factors that influence the incidence of anemia, one of which can be measured through Mid-Upper Arm Circumference (MUAC) as a simple indicator of the body's energy and protein reserves. This study aims to determine the relationship between LISA and the incidence of anemia in third-trimester pregnant women in the working area of the Margasari Community Health Center (UPTD Puskesmas Margasari). This study used an observational analytical design with a cross-sectional approach. A sample of 30 third-trimester pregnant women was selected using purposive sampling. LISA measurements were taken using a non-elastic measuring tape, while anemia status was determined based on hemoglobin levels using WHO standards. Data analysis was performed using Fisher's Exact Test with a significance level of 0.05. The results showed a higher proportion of anemia in pregnant women with LISA < 23.5 cm compared to the group of women with LISA ≥ 23.5 cm. Statistical tests showed a significant relationship between LILA and the incidence of anemia (p = 0.004). The conclusion of this study is that maternal nutritional status based on LILA measurements is significantly related to the incidence of anemia in third trimester pregnant women. The LILA indicator can be used as an early screening tool in antenatal care services to prevent the risk of anemia and pregnancy complications.