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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Ratna Agustina
Clinical Pathology Residency Education Program, Faculty of Medicine Universitas Udayana/Prof. Dr. I.G.N.G Ngoerah General Hospital, Denpasar
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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Concordance Between Sysmex UF-5000 Bacterial Flagging and Urine Culture Results for Differentiating Gram-Positive and Gram-Negative Bacteria Ratna Agustina; Anak Agung Wiradewi Lestari; I Nyoman Wande; Sianny Herawati; Ni Nyoman Mahartini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 32 No. 3 (2026)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v32i3.2589

Abstract

Urinary tract infections (UTIs) are among the most common infectious diseases globally, accounting for approximately 15% of all hospitalizations. Urine culture is considered the gold standard for diagnosing UTIs. However, this method requires specialized equipment and is time-consuming. In recent years, urine analysis by flow cytometry has begun to be used to detect bacteriuria quickly, accurately, and cost-effectively. The Sysmex UF-5000 uses flow cytometry to differentiate Gram-positive and Gram-negative bacteria, providing “BACT” flagging information. This analytical observational study with a cross-sectional design was conducted at the Integrated Laboratory of Ngoerah Hospital, Denpasar, from April to June 2023. Consecutive urine culture samples with sufficient volume (≥3 mL) were collected for urinalysis. The concordance between the Sysmex UF-5000 flagging and urine culture results was analyzed using Cohen's Kappa test. A total of 50 samples were analyzed. This study demonstrated agreement between the Sysmex UF-5000 flagging results and urine culture findings in differentiating Gram-positive and Gram-negative bacteria, with a Cohen's Kappa coefficient of 0.81. The Sysmex UF-5000 flagging shows excellent agreement or substantial concordance with urine culture results in differentiating Gram-positive and Gram-negative bacteria.
Co-Authors Anak Agung Wiradewi Lestari I Nyoman Wande Ni Nyoman Mahartini Sianny Herawati