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All Journal Jurnal Medik Veteriner
Seagames Waluyo
PT Sciencewerke, West Jakarta 11480, Indonesia
Biochemistry, Genetics & Molecular Biology Veterinary

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Preliminary study on Anti-Müllerian hormone (AMH) protein profiling and ovarian biomarkers associated with reproductive status in beef cows Dicky Mohammad Dikman; Pudji Srianto; Aulanni’am; Epy Muhammad Luqman; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Erma Safitri; Tita Damayanti Lestari; Supriyadi; Gabriel Sampe Pasang; Heni Puspitasari; Seagames Waluyo
Jurnal Medik Veteriner Vol. 9 No. 2 (2026): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss2.2026.495-502

Abstract

This preliminary study investigated associations among reproductive status, ovarian diameter, and Anti-Müllerian hormone (AMH)-related protein profiles in beef cows. Fifty postpartum cows were classified into two groups: <60 days postpartum (n = 25) and >60 days postpartum (n = 25), and further categorized by reproductive status. Ovarian diameter was measured by ultrasonography, while serum AMH concentration, total protein level, and protein band patterns were evaluated as candidate biomarkers. Cows with normal reproductive status more frequently had ovarian diameters ≥2.0 cm, whereas all cows with reproductive disorders had diameters <2.0 cm. Cows >60 days postpartum showed significantly higher total protein levels, AMH concentrations, and numbers of protein bands than those <60 days postpartum. Western blot analysis identified a distinct AMH-reactive protein band at approximately 60–70 kDa, representing a potential immunodiagnostic target, although detailed epitope characterization was not performed. These findings suggest that ovarian diameter and circulating AMH may serve as indicators associated with reproductive status and postpartum stage in beef cows. The AMH-reactive band provides preliminary molecular evidence for developing AMH-based immunodiagnostic assays. Further studies should validate specific epitopes, develop targeted antibodies, and evaluate prototype fertility tests for field application.
Co-Authors Aulanni’am Dicky Mohammad Dikman Epy Muhammad Luqman Erma Safitri Gabriel Sampe Pasang Heni Puspitasari Pudji Srianto Sri Pantja Madyawati Supriyadi Tita Damayanti Lestari Tri Wahyu Suprayogi