<![CDATA[Talinum paniculatum (Jacq.) Gaertn., (Javanese ginseng) commonly known as a source of diverse bioactive compounds, leading to increasing demand and the need for efficient propagation techniques to enhance metabolite production. This research examines the influence of nano-TiO2 elicitation on bioactive compound accumulation in T. paniculatum callus cultures. The methods used in the study include callus induction, elicitation, callus extraction, proline quantification, H2O2 analysis, phytochemical profiling and data analysis. The results show that none of the nano-TiO2 treatments significantly affected proline levels, but did have a significant effect on H2O2 accumulation. Phytochemical analysis demonstrated that nano-TiO2 at 2 mg/L increased the proportion of n-Nonadecanol-1 (27.56%), while 3 mg/L enhanced the levels of Nonadecane (10.14%), 1-Tetradecene (11.03%) and 1-Dodecene (315.56%). Nano-TiO2 at 5 mg/L elevated the percentages of 2,4-Ditert-butylphenol (110.98%), Undecane (89.30%), 1-Tetradecene (96.32%), n-Tetracosanol (23.81%) and 1-Heptacosanol (167.79%). The dominant classes of bioactive metabolites identified were fatty alcohols (32.41%), followed by phenolics (21.23%), alkenes (14.87%), alkanes (9.86%), fatty acids (3.44%), phthalate esters (3.02%), sesquiterpenes (2.28%), aromatic ketones (1.89%), phenylpropanoids (0.48%), diterpenes (0.52%) and ketones (0.33%). Overall, the findings indicate that each nano-TiO2 concentration influences the accumulation of different bioactive compounds in T. paniculatum callus cultures.]]>
