Background: Vaginal discharge is a common reproductive health problem among adolescent females and may be either physiological or pathological. If not properly managed, it can lead to complications. One factor suspected to influence the occurrence of vaginal discharge is the improper use of pantyliners, particularly when their use does not adhere to the principles of genital hygiene. Purpose: To determine the relationship between pantyliner use and the occurrence of vaginal discharge among female students, based on theories of reproductive health, personal hygiene, and vaginal flora imbalance. Method: This study employed a quantitative analytical design with a cross-sectional approach. The sample consisted of 87 respondents selected using purposive sampling. Data were collected through a questionnaire that had been tested for validity and reliability and were subsequently analyzed using the Chi-square test. Results: The results showed that most respondents rarely used pantyliners (87.4%), while the majority experienced vaginal discharge (86.2%). Bivariate analysis revealed a significant relationship between pantyliner use and the occurrence of vaginal discharge (p = 0.015). Improper use of pantyliners, such as prolonged use without changing them regularly, may increase moisture in the genital area, thereby promoting the growth of microorganisms that can cause vaginal discharge. Conclusion: In conclusion, there is a significant relationship between pantyliner use and the occurrence of vaginal discharge. Therefore, education regarding the proper use of pantyliners and the practice of good personal hygiene is needed as a preventive measure.