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Wijayani, Bhakti Wisnu
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Agriculture, Biological Sciences & Forestry Environmental Science

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Studi Hifa Eksternal CMA dalam Memahami Fungsinya Mengkontribusi Serapan P Tanaman Menggunakan Metode Thin Section Effendy, Machfud; Wijayani, Bhakti Wisnu
JOURNAL OF TROPICAL SOILS Vol. 13 No. 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.241-252

Abstract

The study of the external hyphae of AMF was conducted for getting data of the soil hyphae distribution from the observation using thin section preparation. The experiment was arranged in factorial fully randomized design. The 1st factors are dosages of P application: 0; 45; 90; 135; and 180 kg ha-1.  The second factor was AMF spore inoculation: with inoculation and without inoculation.  The observations were conducted to roots and shoots oven dried, absorption of P, P32- fertilizer and P31 from soil.  The soil was separated from T-pots and to prepare for making the thin section of soil for observation to hyphae distribution at every 2 cm level.  The experiment results showed that the prepared of thin section was documented at the 200 µm x 200 µm (= 40.000 µm2) dimension can be used for external hifas observation.  In the 400 µm2 areas of soil without AMF inoculated has about 24 to 27 external hyphal, so at the  40.000 µm2 areas has 2.400 until 2.700 hyphae.  The soil was AMF inoculated has 19 to 25 hyphae at  400 µm2 areas of soil, and in the 40.000 µm2 areas has  1.900 until 2.500 hyphae and the length of hyphae about 67-75 m g-1.  The diameter hifas at soil without AMF inoculation about 8-10 µm, and at the soil with AMF inoculated has hyphae diameter about 8-11 µm, and the long of hyphae about 53-69 m g-1.  The growth speed of hyphae about 0.74-0.89 m day-1 or about 0.031-0.035 m hour-1.  The inoculated plant with AMF spore gave more in contribution to P32 fertilizer and soil P31 than that in uninoculated plant, but the yield of shoot and root oven dried was higher for plants without inoculated AMF spore.  The inoculated soil with spore of AMF caused to sum of AMF spore and soil P availability was higher than that in uninoculated soil.
Estimation of Available Phosphorus in Soil Using the Population of Arbuscular Mycorrhizal Fungi Spores Effendy, Machfud; Wijayani, Bhakti Wisnu
JOURNAL OF TROPICAL SOILS Vol. 16 No. 3: September 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i3.225-232

Abstract

Soil microbes, such as arbuscular mycorrhizal fungi (AMF) have the ability to dissolve unavailable phosphorus (P)  and they can be used as an indicator of the P availability in soil. The study was conducted on upland soil  in East Java. The soil  was sampled twice, before and after planting at the harvesting time. The population of AMF spores and soil P availability were observed. The AMF spores were isolated using wet sieving method, decanting, and followed by sucrose density gradient centrifugation. The available P was observed using the Olsen extraction. The numbers of AMF spore was corelated with available P, moreover the numbers of AMF spore was compared to the availabality of P.  The results showed that the total number of AMF spores at six sites were ranged from a little to midle, and the available P ranged from low to high level. All soil site samples had a linear corelation between numbers of AMF spore and  available P in soil.  The greater the number of AMF spore, the higher the available P in soil. It was likely that the availability of P in soil can be predicted by  the population of AMF spores in soil. Therefore, the number of AMF spore can be need as a biological method to predict the available P  in soil and to make a recommendation the use of P fertilizer.
Co-Authors Effendy, Machfud