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All Journal Media Penelitian dan Pengembangan Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Rahayu, Sri Hartin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Public Health

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KEMAMPUAN BACILLUS LICHENIFORMIS DALAM MEMPRODUKSI ENZIM PROTEASE YANG BERSIFAT ALKALIN DAN TERMOFILIK Soeka, Yati Sudaryati; Rahayu, Sri Hartin; Setianingrum, Ninu; Naiola, Elidar
Media Penelitian dan Pengembangan Kesehatan Vol 21, No 2 Jun (2011)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v21i2 Jun.109.

Abstract

The aim of the research was to measure the production of protease enzyme in alkaline and thermophilic of bacteria Bacillus licheniformis. The protease enzyme signed by clear zone arround the bacteria colonies on medium which contain 1% skimmed milk. The activities of protease enzyme treated  by the period of incubation, temperature and pH, which measured by spectrophotometer at ? 280 nm. The results showed that the highest production of protease activity at 2 days incubation was 150,52 U/mL. At temperature 50°C and pH 10 they were 123,34  U/mL and 193,14 U/mL.
Analisis Ekspresi Gen Selenometil Transferase pada Isolat Bakteri Termofilik Geobacillus 20K dan Thermomicrobium 14Ka sebagai Sumber Selenoprotein Triana, Evi; Nurhidayat, Novik; Rahayu, Sri Hartin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.614 KB) | DOI: 10.24002/biota.v14i3.2580

Abstract

Selenium is a trace element that has essential nutrition value for human. Besides its nutritional value, it has important health benefits, including being a cancer chemoprotective agent. Methylated form of selenium is the most effective compound against cancer cells. Selenomethyl transferase (SMT) is responsible for methylating of selenium. This enzyme is coded by selenomethyl transferase (smt) gene which was found only from selenium accumulator plant, Astragalus bisulcatus. Thermophilic bacteria Thermomicrobium 14Ka and Geobacillus 20K have ability to accumulate selenium as well and potential in fighting cancer cells. Therefore a study to determine smt gene and its expression in both bacteria had been conducted in order to develop natural product of seleno-metilselenosistein for cancer treatment. The result showed that Thermomicrobium 14Ka and Geobacillus 20K have putative smt (selenomethyl transferase) gene, and such gene was expressed at different intensity. Geobacillus 20k expressed smt gene at higher intensity than Thermomicrobium 14k. Therefore, it is presumable that Geobacillus has a significant role in cancer remedy, meanwhile Thermomicrobium plays an essential role as cancer protective agent.
Co-Authors Evi Triana Naiola, Elidar Novik Nurhidayat Setianingrum, Ninu Yati Sudaryati Soeka