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Keisha Rasheda
Universitas Aisyiyah Yogyakarta
Humanities Engineering Health Professions Nursing Public Health

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Effect of hemolysis index on serum magnesium levels: an in vitro experimental study Yeni Rahmawati; Arifiani Agustin Amalia; Salsabila Amalia; Keisha Rasheda
International Journal of Health Science and Technology Vol. 8 No. 1 (2026): July
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/ijhst.v8i1.4967

Abstract

Hemolysis is one of the preanalytical factors that can affect the accuracy of laboratory test results, including serum magnesium levels. This study aimed to determine the effect of the serum hemolysis index on magnesium levels in in vitro samples. This experimental study consisted of four groups: normal serum as the control group and serum samples with hemolysis indices of 20 mg/dL, 40 mg/dL, and 80 mg/dL, with six samples in each group. The results showed that the mean magnesium levels were 2.30 ± 0.20 mg/dL in normal serum, 2.48 ± 0.32 mg/dL in the 20 mg/dL hemolysis group, 2.62 ± 0.30 mg/dL in the 40 mg/dL hemolysis group, and 4.12 ± 0.79 mg/dL in the 80 mg/dL hemolysis group. The Shapiro-Wilk normality test indicated that the data were normally distributed, whereas the Levene test showed unequal variances among the groups. One-way analysis of variance (ANOVA) demonstrated a significant difference among the groups (p = 0.001), which was confirmed by the Kruskal-Wallis test (p = 0.002). The Tukey HSD post hoc test showed that the 80 mg/dL hemolysis group had significantly higher magnesium levels than the other groups, whereas the 20 mg/dL and 40 mg/dL hemolysis groups did not differ significantly from the normal serum group. In conclusion, hemolysis, particularly at an index of 80 mg/dL, significantly increases serum magnesium levels and may result in biased laboratory test results.
Co-Authors Arifiani Agustin Amalia Salsabila Amalia Yeni Rahmawati