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All Journal Jurnal Ilmu Peternakan Indonesia (JIPENA)
Teguh Sumarsono
Universitas Jambi
Agriculture, Biological Sciences & Forestry

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Pengaruh Lama Pendinginan Sebelum Ekuilibrasi Terhadap Kualitas Spermatozoa Sapi Simmental Pasca Ekuilibrasi Nur Hayulin Inayah; Fachroerrozi Hoesni; Teguh Sumarsono
Jurnal Ilmu Peternakan Indonesia Vol. 3 No. 3 (2026): JIPENA - Oktober
Publisher : CV. SINAR HOWUHOWU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70134/jipena.v3i3.2088

Abstract

The purpose of this study was to determine the effect of pre-equilibration cooling time on the post-equilibration quality of Simmental bull spermatozoa. This research was conducted at the Laboratory of the Regional Technical Implementation Unit (UPTD) of the Livestock Breeding Center, Office of Food Crops, Horticulture, and Animal Husbandry of Jambi Province from October 10 to November 10, 2025. An experimental method was used with a completely randomized design (CRD) consisting of four treatments and six replications. The treatments included P0 (2 hours of pre-equilibration cooling), P1 (4 hours of pre-equilibration cooling), P2 (6 hours of pre-equilibration cooling), and P3 (8 hours of pre-equilibration cooling). The results showed that the pre-equilibration cooling time significantly affected the motility, viability, and membrane integrity of Simmental bull spermatozoa (P < 0.05). It can be concluded that the optimum pre-equilibration cooling time to produce good quality Simmental bull spermatozoa is 2 to 4 hours.  
Kualitas Semen Beku Sapi Simmental Dengan Metode Thawing Yang Berbeda Muhammad Firman Saputra; Fachroerrozi Hoesni; Teguh Sumarsono
Jurnal Ilmu Peternakan Indonesia Vol. 3 No. 3 (2026): JIPENA - Oktober
Publisher : CV. SINAR HOWUHOWU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70134/jipena.v3i3.2129

Abstract

This study aims to determine the effect of different thawing methods on the post-thaw quality of Simmental cattle frozen semen, covering spermatozoa motility, viability, and abnormality. The research was conducted at the Laboratory of UPTD Balai Pembibitan Ternak, Department of Food Crops, Horticulture, and Livestock of Jambi Province. The material used consisted of 20 straws of Simmental frozen semen. The study used a Completely Randomized Design (CRD) with 4 treatments and 5 replications. The treatments applied were P1 (31°C for 30 seconds), P2 (34°C for 25 seconds), P3 (37°C for 20 seconds), and P4 (40°C for 15 seconds). The observed variables included spermatozoa motility, viability, and abnormality. The data were analyzed using Analysis of Variance (ANOVA), and if there was a significant effect, it was followed by Duncan's Multiple Range Test. The results showed that the thawing method had a significant effect (P<0.05) on spermatozoa motility and viability, but had no significant effect (P>0.05) on spermatozoa abnormality. The best treatment was obtained in P3 (37°C for 20 seconds) with a motility value of 54,00 ± 1,17%, viability of 64,60 ± 0,65%, and the lowest abnormality of 5,20 ± 0,57%. Treatment P4 (40°C for 15 seconds) resulted in the lowest motility and viability, while the percentage of abnormality across all treatments remained within the normal range for semen suitable for artificial insemination. Based on the results, it can be concluded that the thawing method at a temperature of 37°C for 20 seconds yields the best post-thaw quality of Simmental cattle frozen semen.  
Co-Authors Fachroerrozi Hoesni, Fachroerrozi Muhammad Firman Saputra Nur Hayulin Inayah