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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL BIOLOGI INDONESIA
Panggabean, Lily
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Pseudo-Nitzschia pseudodelicatissima Isolated From Hurun Bay: Salinity Tolerance and Domoic Acid Content Panggabean, Lily
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.763 KB) | DOI: 10.24002/biota.v12i1.2535

Abstract

Pseudo-nitzschia pseudodelicatissima dari sampel perairan Teluk Hurun, Lampung berhasil diisolasi dan dikultur dalam medium F/2 dengan penyinaran 2500 luks 12 jam per hari pada suhu 24-250C. Perlakuan salinitas menunjukkan bahwa P. pseudodelicatissima tidak toleran terhadap salinitas kurang dari 15 PSU dan sangat toleran terhadap salinitas yang tinggi hingga mencapai 45 PSU (Pressure Salinity Unit). Salinitas optimum untuk pertumbuhan P.pseudodelicatissima yaitu pada 30 dan 35 PSU. Analisis ekstrak P. pseudodelicatissima, 9 hari sesudah inokulasi, menggunakan HPLC-UV tidak menunjukkan adanya kandungan asam domoat.
EKSTRASI SENYAWA ANTIBAKTERI DARI DIATOM CHAETOCEROS GRACILIS DENGAN BERBAGAI METODE Setyaningsih, Iriani; Hardjito, Linawati; Monintja, Daniel R.; Sondita, M. Fedi A.; Bintang, Maria; Lailati, Nispi; Panggabean, Lily
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3203

Abstract

ABSTRACTExtraction of Antibacterial Compound from Diatom Chaetoceros gracilis WithDifferent Methods. Diatom is phytoplankton which is commonly found in off shore.Chaetoceros produce antibacterial which inhibit some bacteria. This research was donein 3 steps. At first, Chaetoceros gracilis was cultivated in temperate controlled roomwith lighting 24 hours. The culture was harvested on 14 days, then it was dried andweighted. The second step, biomass was disrupted by different method such as sonicator,glass beads, and undisrupting. Then the biomass was maserated, filtrated, and evaporated.The crude extracts were tested to pathogen bacterial. The third step, the extraction wasconducted using hexana (non polar solvent), ethyl acetate (semi polar solvent), andmethanol (polar solvent). The crude extracts were tested to the pathogenic bacteria.The result showed that the produce cell disruption antibacterial activity by sonicator.The biggest inhibition zone was obtained by hexana but produced lower yield.Key words: Chaetoceros gracilis, growth, extraction, disrupting, antibacteria
Co-Authors Daniel R. Monintja Lailati, Nispi LINAWATI HARDJITO M. Fedi A. Sondita MARIA BINTANG