Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Annales Bogorienses
Ines I. C. Atmosukarto
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Investigation of Functional Difference between TecIII and TecIV in Mammals COS-1 Cells Using GFP Fusion Proteins Ines I. C. Atmosukarto; Grant W, Booker
Annales Bogorienses Vol. 10 No. 1 (2005): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2005.v10.n1.1-7

Abstract

Signal transduction cascades are critical components of intra-and inter-cellular communication. Key component of such cascades includes tyrosine kinases. One such family of tyrosine kinase is the Tec family of tyrosine kinases. This family of tyrosine kinases is expressed mainly in cells of the hematopoietic lineage, and mutations in at least one of its one member of this family, Btk, has so far been associated with the human immunodeficiency disorder X-Linked Agammaglobulinemia. Two major isoforms of the Tec transcript, referred to as TecIII and TecIV have been detected in various mouse embryonic and adult tissues, as well as in a number of different hematopoietic cell lines: TecIV is the full length Tec protein with functional PH, TH, SH3, SH2, and Kinase domains, while TecIII is gen rated by the splicing out of exon 8 sequences to yield a shorter peptide with a non-functional SH3 domain. Using GFP-TecIII fusion proteins, this shorter isoform of Tec was shown to have biological characteristics that differed from TecIV.
The Development of A Bioassay Based on Heterologous Expression of M2 Ion-Channel Protein Anggia Prasetyoputri; Neti Yuliaty; Warda Tuharea; Alisin Febyanti; Bambang Sunarko; Ines I. C. Atmosukarto
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2010.v14.n2.9-14

Abstract

Emerging resistant viral strains combined with the limited availability of antivirals in a pandemic scenario highlight the need for the development of novel influenza antivirals. A bioassay based on the M2 protein of influenza virus - a potential target for antivirals - was developed to screen endophytic microbial extracts. M2 can be synthesized using PCR, thus eliminating the need for the handling of infectious specimen. Following cloning of the M2 gene into a pET backbone, the resultant plasmid was transformed into BL21 (DE3) pLyss E. coli cells. Cultures of these cells were set up at 37C following inoculation with a starter culture, to reach an OD at 600nm (OD600) of 0.4-0.6. Once at the required OD, the culture was split in two aliquots and expression of the M2 protein was induced in one of the duplicates with the addition of isopropyl β-D-thiogalactopyranoside (IPTG). Bacterial growth was monitored at 60-minute intervals. Exogenous expression of the M2 protein has been reported to decrease host cells viability, resulting in lower OD600 values. Our results suggest that the M2 protein was expressed and that overexpression of this protein resulted in consistently lower OD600 values of induced cultures compared with that of uninduced cultures. Based on this principle, extracts can be screened for their ability to block M2 function as identified by increased OD600 values.
Co-Authors Alisin Febyanti Anggia Prasetyoputri Bambang Sunarko Grant W, Booker Neti Yuliaty Warda Tuharea