Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Annales Bogorienses
Andi Utama
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Construction of A Recombinant Virus Between Poliovirus and Coxsackie A Virus 11 Andi Utama; Hiroyuki Shimizu
Annales Bogorienses Vol. 10 No. 1 (2005): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2005.v10.n1.15-22

Abstract

Recent outbreaks of circulating vaccine-derived poliovirus (cVDPV) revealed the possibility of recombination between vaccine strains poliovirus (PV) and cluster C enterovirus. Based on genetic analysis, it is assumed that coxsackie A virus 11 (CAV-11), one f the cluster C enterovirus, may naturally recombine with PV. To elucidate this hypothesis, the chimeric virus between PJ156, a type I cVDPV isolate isolated from an acute flaccid paralysis (AFP) case in the Philippines in 2001, and CAV-11 (PJ156/CAV-11) was constructed by using long-PCR method. As the result , PJ156/CAV-11 was viable in HEp-2 cell line. The PJ156/CAV-11 exhibited mostly similar phenotype with parental PJ156 in term of plaque size, viral growth and neuroviruJence. These results suggested that recombination between PV and CAV-11 might naturally occur during transmission of vaccine strains in the community. The effect of recombination on the viral phenotype is significantly depending on the counterpart virus.
Motif II of Japanese Encephalitis Virus NS3 Protein is Not Essential for RNA Binding Activity Andi Utama; Hiroyuki Shimidzu
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2007.v11.n1.15-21

Abstract

The role of motif II (DEAH; Asp285-Glu286-Ala287-His288) of Japanese encephalitis virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to the motif and the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL21(DE3)pLysS and purified by metal affinity resin. Asp-285 and Glu-286 was respectively substituted with Ala. Ala-287 was replaced by Cys, Gly, or Ser. His-288 was mutated to other 19 amino acids. In total, 24 mutant proteins were produced and analyzed. As results, all mutants showed quite similar RNA binding activity indicating that motif II of JEV NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C viruc (HVC) NS3 protein, suggesting lhe similar structure of NS3 protein in the flavivirus.
Co-Authors Hiroyuki Shimidzu Hiroyuki Shimizu