Maria Imelda
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)

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In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Maria Imelda; Aida Wulansari; Sumarnie Sumarnie
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.39-43

Abstract

Pandanus conoideus Lam or 'Buah merah' of the Pandanaceae is a native to East Indonesia, particularly Papua and North Maluku. Traditionally the fruits are used for health promotion and maintenance as well as for curing several illnesses. Recently, it has been reported that the fruit its are potential for cancer medication. As a result, there has been an overexploitation of the plants from their habitats. In order to anticipate their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation, and cultivation of these plant should be developed. Generally buah merah is propagated vegetatively by offshoots and stem cuttings or generatively by seeds. Micropropagation has many advantages over the conventional method because the technique allows mass clonal and pathogen-free production of plants at a high rate of multiplication all year round. In this research the effects of 0.1-0.2 mg/L thidiazuron (TDZ), 0.5-1.0 mg/L benzyl amino purine (BAP), and 0.25-0.5 mg/L kinetin (KN) on shoot bud induction and proliferation of P. conoideus were investigated using nodal infections or lateral buds of P. conoideus on modified Murashige and Skoog (MS) medium. Shoots were rooted on MS medium without plant growth regulator (PGR). The result showed that lateral bud of Pandanus start d to initiate growth after 4-7 days in culture. The best medium for shoot proliferation was MS containing either 0.5 mg/l BAP with 0.1 mg/l TDZ or 1 mg/l BAP with 0.5 mg/l KN giving a multiplication rate of 16.5 shootlets per shoot bud explant after 8 weeks. Rooting of shoots was successfully conducted on MS medium without PGR. Acclimatization of rooted plantlets was achieved on a mixed medium of cocopeat and soil (1:1).
Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) Through In Vitro Shoot Proliferation Maria Imelda; Aida Wulansari; Laela Sari
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2009.v13.n1.21-28

Abstract

Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to the Asia-Pasific region. Beside its delicious fruit, sukun is also known as a traditional herbal medicinal plant in the region, including Indonesia. Nearly all parts of the plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease. However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun is propagated by root or stem cuttings, since in Indonesia sukun does not produce any seeds. However such method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal, free from pest and diseases, more uniform, and allowing a high rate of plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of 1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS)on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water (CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks.