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Dwi Widyajayanti
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)

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Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Satya Nugroho; Vincentia Esti Windiastri; Dwi Widyajayanti; Carla Frieda Pantouw
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2009.v13.n1.29-36

Abstract

Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority. The screening protocol based on PEG 6000 has been developed and was being used to screen 70 mutant lines tocharacterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds). These characters were used to score the Degradation Index and Vigour Index. Results showed varyingresponds of the lines to the osmotic pressure. Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified. Some inconsistencies in the performances scored by both indices were thought to be due to seed quality.
Phenotypic Screening of Ds Transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Satya Nugroho; Anky Zannati; Apriadi Situmorang; Vincentia Esti Windiastri; Dwi Widyajayanti; Carla Frieda Pantouw; Dwi Astuti; Sri Indrayani; Syamsidah Rachmawati; Enung Sri Mulyaningsih; Tri Joko Santoso; Kurniawan Rudi Trijatmiko
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2011.v15.n1.21-25

Abstract

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller. 
Co-Authors Anky Zannati Apriadi Situmorang Carla Frieda Pantouw Dwi Astuti Enung Sri Mulyaningsih Kurniawan Rudi Trijatmiko Satya Nugroho Sri Indrayani Syamsidah Rachmawati Tri Joko Santoso Vincentia Esti Windiastri