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Tutik Murniasih
Research Center for Oceanography, Indonesian Institute of Sciences (LIPI)

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The Investigation of Polycyclic Aromatic Hydrocarbon and Oil Degrading Bacteria Isolated from The Marina Port Ancol, Jakarta Bay Puspita Lisdiyanti; Yopi Yopi; Tutik Murniasih
Annales Bogorienses Vol. 15 No. 2 (2011): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2011.v15.n2.17-23

Abstract

Polycyclic Aromatic Hydrocarbons (PAHs) as well as crude oil are widespread environmental pollutants. The contamination of air, soil, freshwater (surface water and groundwater), and marine environments by PAHs as well as crude oil has been reported. Of concern to public health is the fact that many PAHs or their metabolites are mutagenic, carcinogenic, or both. North Java coastal area such as Jakarta Bay is the polluted marine area in Indonesia as a result from anthropogenic wastes and the oil spill. Although evaporation and photo-oxidation play an important role in oil detoxification, ultimate and complete degradation is accomplished mainly by marine micro flora, and being dominant in this function. Certain bacteria are well-known could consume and degrade the PAHs as well as crude oil. Therefore investigating the potential PAH and oil degrading marine bacteria is important. In this study, we collected sample from oil polluted area in Marina Port Ancol, Jakarta Bay and isolated four PAH substrates and Arabian crude oil degrading marine bacteria using enrichment method and direct isolation method. As result, 223 strains could degrade PAHs, among these strains, 94 strains could degrade phenanthrene, 23 strains degrade fluoranthene, 92 strains could degrade dibenzotiophen, 14 strains could degrade phenotiazin and 106 isolates degrade crude oil.
Antioxidant Capacities of Holothuria Sea Cucumbers Tutik Murniasih; Masteria Yunovilsa Putra; Ratih Pangestuti
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2015.v19.n2.21-26

Abstract

Sea cucumbers are a potential source of biologically active metabolites that are widely used for nutraceutical, pharmaceutical and cosmeceutical products. In this study we investigated the antioxidant activity of several extracts of sea cucumbers, those are Holothuria scabra, Holothuria atra, Holothuria leucospilota, and Holothuria excellens collected from Lombok Island. The selected sea cucumber was then separated to get the active fractions and identified for profiling the secondary metabolites. Phytochemical screening reveals the presence of flavonoid, terpenoids, phenol, saponin, alkaloid, anthraquinone, and glycoside in the extracts of Holothuria sea cucumbers. Radical scavenging effects on the DPPH (1,1-diphenyl-2-picryl-hydrazyl) model were evaluated. The previous work reported that the crude extract of H. atra showed the highest potential of antioxidant capacity. H. atra extract was separated by column chromatography on silica gel and eluted with a gradient system of increasing polarity. Fraction 7 of H. atra extract showed significantly inhibited radical scavenging activity (35.3487 %) at concentration 1 mg/ml. The result of Gas Chromatography-Mass Spectrometry analysis of H. atra fraction 2 showed that it has several active antioxidant compounds which are ethyl cetylate (3), hexadecyl-oxirane (5), 3-chloro-4-hydroxybenzoic acid (6), andoleyl alcohol, trifluoroacetate (9). Moreover, the active fraction 7 indicated several compounds, those are cycloicosane (17), pentadecyl trichloroacetate (18), 14b-pregnane (21) and cis-4-yano-2-(2-hydroxycyclohexyl) pyridine. However, these are preliminary findings and further studies are needed to be focus on the purifications of unknown compounds and formulation for nutraceuticals.
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Nina Artanti; Faiza Maryani; Hanny Mulyani; Rizna Triana Dewi; Vienna Saraswati; Tutik Murniasih
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2016.v20.n1.25-30

Abstract

Currently marine bacteria are considered as important source of natural products for drug discovery. The objective of this study is to conduct an in vitro bioactivities (antidiabetic, antioxidant and antibacterial) screening of 9 Indonesian marine bacteria isolated from sponges that belongs to the Research Center for Oceanography, Indonesian Institute of Sciences collections. The marine bacteria were cultured for 2 days in liquid medium containing yeast, peptone and sea salt under shaking condition and extracted with ethyl acetate. Antidiabetic was measured using inhibition of -glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method. Screening results showed that at sample concentration of 200 μg/mL, there was significant -glucosidase inhibitory activity detected in the extracts of strain Sp 7.9 (84 % inhibition) and Sp 8.10 (75 % inhibition), however the antioxidant activities of these two strains were low only around 30 % inhibition, antioxidant activities of other strains were very low. Screening for antibacterial activities using 10 μL samples show that extract of strain Sp 8.5 was best for Staphylococcus aureus (14 mm inhibition); Sp 7.9, and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 (10 mm inhibition) for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetic and antibacterial. Results of molecular identification conducted by InaCC show that identity of both strains based on BLAST Homology using NCBI database were Bacillus thuringiensis.
Co-Authors Faiza Maryani Hanny Mulyani Masteria Yunovilsa Putra Nina Artanti Puspita Lisdiyanti Ratih Pangestuti Rizna Triana Dewi Vienna Saraswati Yopi Yopi