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All Journal Annales Bogorienses
Maggy Thenawidjaja Suhartono
Bogor Agricultural University

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Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Wendry Setiyadi Putranto; Kusmajadi Suradi; Hartati Chairunnisa; Apon Zaenal Mustopa; Harsi Dewantari Kusumaningrum; Maggy Thenawidjaja Suhartono
Annales Bogorienses Vol. 21 No. 1 (2017): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2017.v21.n1.9-14

Abstract

Rennin-like protease of many microorganisms behave similarly to chymosin and are potential alternatives of rennet. The lactic acid bacteria with milk clotting activity were isolated from Bakasam, an Indonesian traditional fermented meat. Screening assay was carried out using modified method of skim milk agar and milk clotting activity test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/mL. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% identical with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.
Co-Authors Apon Zaenal Mustopa Harsi Dewantari Kusumaningrum Hartati Chairunnisa Kusmajadi Suradi Wendry Setiyadi Putranto