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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA)
Arasy, Andi Arahmadani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology Physics

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Ovitrap Index and Transovarial Transmission Rate of Dengue Virus of Male and Female Aedes aegypti Mosquitoes in Makassar, South Sulawesi, Indonesia Juhdi, Isnadiyah; Fitri, Loeki Enggar; Zuhriyah, Lilik; Arasy, Andi Arahmadani
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.524 KB) | DOI: 10.11594/jtls.09.01.13

Abstract

The incidence rate (IR) of dengue hemorrhagic fever (DHF) in Indonesia has increased in recent years and still causes a health problem. Makassar, as one of the big cities in Indonesia, is also experiencing of DHF cases. According to the Health Office of Makassar City, DHF cases during 2014 – 2016 has increased from 139 to 250 cases. The defense mechanism of dengue virus in transovarial transmission from female mosquitoes to eggs is estimated to be one of the factors that increase the rate of DHF. In addition, the circulation of the Aedes aegypti mosquitoes can also affect the increase of the rate. This study aims are to identify ovitrap index and to find out how much the vertical transmission of dengue virus by measuring the minimum infection rate (MIR) and the distribution of four dengue virus serotype in Ae. aegypti mosquitoes in the city of Makassar, South Sulawesi, as well as their association with the incidence rate (IR) in the area. This observational research uses ecological study with cross-sectional approach. The samples of the study are male and female mosquitoes from Ae.aegypti eggs obtained from the installation of ovitrap  in 30 urban villages in Makassar. Dengue virus identification was performed using a reverse transcriptase polymerase chain reaction (RT-PCR). These results showed that the ovitrap index (IO) was in the range of 0 - 66.7%. The average value of the ovitrap index outside the house (44.44%) was greater than inside house (37.22%). The results of identification of dengue virus using RT-PCR showed negative results as indicated by the non-formation of a band on 75 pools male and 78 pools female Ae. aegypti mosquitoes after electrophoresis. The results of this study indicated that the Ae. aegypti mosquito in Makassar City showed a tendency to lay eggs outside the house due to the habit of the people who put rain water shelters outside the house which could become breeding place for Ae. aegypti mosquitoes. This study also showed that there was no transovarial transmission of DENV found in male and female Ae. aegypti mosquitoes in Makassar City during November 2017 until January 2018.
Analisis Faktor Lingkungan yang Mempengaruhi Keberadaan Tikus Positif Bakteri Leptospira sp. di Kota Kendari Arasy, Andi Arahmadani; Nurjazuli; Adi, M. Sakundarno
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.5441

Abstract

Rats have the potential to transmit Leptospirosis to humans. The incidence of leptospirosis in Sulawesi Province in 2017 there were 2 suspected cases of leptospirosis in Silea Village, Buke District, Kab. South Konawe, which is directly adjacent to the city of Kendari, and the leptospirosis suspect finally died. In 2022, suspected cases of leptospirosis will increase again to 4 cases, this data is based on laboratory examinations which show positive leptospirosis in Kendari City. The aim of the research is to determine environmental factors that influence the presence of mice, including the presence of rubbish, temperature, air humidity, standing water, sewer conditions and identification of Leptospira sp bacteria in caught mice. The design of this research is cross sectional. Collecting the results of observations and examination of leptospira bacteria in rat kidneys was carried out using Portable PCR, while determining the type of leptospira serotype was carried out using the MAT (Microscopic Agglutination Test). The results of this study found that the variable temperature (ρ = 0.014), the variable humidity (ρ = 0.035), the variable the presence of rubbish and the condition of the sewers (ρ = 0.000) which can be interpreted as that there is a relationship between several environmental factors and the presence of mice positive for Leptospira sp bacteria. . Meanwhile, for the waterlogging variable (ρ = 0.281), it was concluded that there was no relationship between waterlogging and the presence of mice and positive Leptospira sp bacteria. There is a relationship between temperature, humidity, the presence of rubbish, and the condition of the sewers and the presence of mice positive for leptospira sp bacteria. in Kendari City in 2023. People in Kendari City should pay more attention to the cleanliness of their living environment.
Co-Authors Juhdi, Isnadiyah Lilik Zuhriyah Loeki Enggar Fitri Mateus Sakundarno Adi, Mateus Sakundarno Nurjazuli