<![CDATA[Antioksidan merupakan senyawa yang dapat menghambat aktivitas radikal tersebut. Enzim superoksida dismutase (SOD) dapat mencegah pembentukan radikal bebas. Tanaman stroberi (Fragaria x ananassa Duchesne) merupakan salah satu tanaman yang memiliki aktivitas enzim SOD. Penelitian ini bertujuan untuk mengetahui aktivitas enzim superoksida dismutase dalam ekstrak daun stroberi sebelum pemurnian dan sesudah pemurnian. Penelitian ini menggunakan sampel daun stroberi. Tahap penelitian diawali dengan determinasi tanaman lalu ekstraksi enzim dengan PBS dan disentrifugasi. Pemurnian enzim menggunakan presipitasi amonium sulfat 25%, 50%, dan 75% dan dialisis. Penetapan kadar protein total ekstrak kasar dan ekstrak hasil dialisis menggunakan metode Lowry dan pengujian aktivitas enzim SOD ekstrak kasar dan ekstrak hasil dialisis dengan menggunakan metode Riboflavin-Nitroblue Tetrazolium (RB-NBT) selanjutnya dianalisis one-way ANOVA. Hasil penelitian menunjukkan ekstrak enzim daun stroberi memiliki kadar protein total sebesar 13,558 mg/mL dan ekstraks enzim hasil dialisis konsentrasi amonium sulfat secara berturut-turut adalah 4,080 mg/mL; 8,174 mg/mL dan 14,806 mg/mL. Nilai persen inhibisi ekstrak kasar daun stroberi sebesar 17,885%, ekstrak hasil dialisis berturut-turut sebesar 20,821%; 26,015%; dan 72,086%. Kata kunci : Antioksidan, fragaria x ananassa duchesne, RB-NBT, superoksida dismutase. Antioxidants are compounds that can inhibit the activity of these radicals. Superoxide dismutase (SOD) enzymes can prevent the formation of free radicals. The strawberry plant (Fragaria x ananassa Duchesne) is one of the plants that has SOD enzyme activity. This study aims to determine the activity of the superoxide dismutase enzyme in strawberry leaf extract before and after purification. This study used strawberry leaf samples. The research stage begins with plant determination and then enzyme extraction with PBS and centrifuge. Enzyme purification uses 25%, 50%, and 75% ammonium sulfate precipitation and dialysis. The determination of the total protein content of crude extract and dialysis extract was determined using the Lowry method and the testing of SOD enzyme activity of crude extract and dialysis extract using the Riboflavin-Nitroblue Tetrazolium RB-NBT method was then analyzed by one-way ANOVA. The results showed that the enzyme extract of strawberry leaves had a total protein content of 13.558 mg/ml and the enzyme extract from dialysis with ammonium sulfate concentration was consecutively, 4.080 mg/mL 8,174 mg/mL; 14,806 mg/mL. The percentage value of inhibition of crude extract of strawberry leaves was 17.885%, and the extract from consecutive dialysis was 20.821%; 26,01%5; and 72,086%. Keywords: Antioxidants, fragaria x ananassa duchenes, RB-NBT, superoxide dismutase]]>
