<![CDATA[This study aimed to develop and validate an evaluation tool for a video demonstration–based General Biology laboratory activity on cellular respiration using feedback from Grade 12 Science, Technology, Engineering, and Mathematics (STEM) students at Holy Angel University during School Year 2021–2022. Students’ perceptions of facility of use, satisfaction, and self-reported laboratory competencies were examined to support instrument construction. A total of 260 Grade 12 STEM students enrolled in General Biology, all with prior exposure to laboratory-based science instruction, participated in the study. A quantitative evaluative research design was employed. The instrument consisted of two sections: (1) three open-ended questions capturing students’ learning experiences, and (2) 26 Likert-type items measuring science process skills adapted from Safaah et al. (2017). A pilot test was conducted among Grade 12 General Academic Strand (GAS) students before administration to the main sample. Descriptive statistics, including means, standard deviations, and percentages, were used to summarize responses. Construct validity was assessed using the Kaiser–Meyer–Olkin (KMO) Measure of Sampling Adequacy and Bartlett’s Test of Sphericity, while internal consistency reliability was examined using Cronbach’s alpha. Results showed acceptable levels of facility, satisfaction, and competency based on predefined scale descriptors. Factor analysis yielded two components with a KMO value of 0.91, indicating excellent sampling adequacy. Items 1, 3, 4, and 15 were removed due to low communalities and weak factor loadings, improving construct clarity. The final instrument demonstrated high reliability (α = 0.92, pilot; α = 0.97, post-test), supporting its validity for evaluating video-based biology laboratory activities.]]>
