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All Journal JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya
Maulida Fajriyah
Departemen Gizi, Sekolah Tinggi Ilmu Kesehatan KHAS, Cirebon, Indonesia
Biochemistry, Genetics & Molecular Biology

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Modifikasi Screen Printed Carbon Electrode Menggunakan Cerium dan Optimasi Kondisi Percobaan Biosensor Elektrokimia untuk Deteksi DNA Mitokondria Sus Scrofa Maulida Fajriyah; Yenni Wahyuni Hartati; Anni Anggraeni; Shabarni Gaffar; Yusuf Rohmatullah
JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya Vol. 6 No. 1 (2022)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um0260v6i12022p031

Abstract

The use of cerium in sensor applications has been widely develop. In this research, electrochemicalbiosensors have been developed to support Sus scrofa mitochondrial DNA using SPCE-Ce. The surface ofthe SPCE electrode before and after an electrodeposition with cerium, was characterized using SEM andcyclic voltammetry (CV). The design of pork nucleotide sequence as DNA probes, was determined usingNCBI blast program and T-Coffee. The DNA probe was immobilized to SPCE-Ce trough electrostaticinteractions between the surface of cerium and the DNA phosphate backbone. The probe-target DNAhybridization was characterized using DPV. The result of the characterization using SEM showed a flat anduniform of electrode surface for SPCE-Ce than the bare SPCE surface. The voltammogram cyclic shows anincrease in the peak of the ferrycianide current for SPCE-Ce. The result of the DNA probe sequence designare 5’-TATTIATACCAATCACTAIC-3’ which shows 100% homology with pig DNA and 0% homology withchicken and beef DNA. The linear equation of target concentration is I = 0.0303 [target DNA] + 3.947 forthe target DNA concentration range 5 – 30 µg/mL, and the LOD value is 1.44 µg/mL. This biosensor can be used to determine pig mtDNA concentration in the real samples
Co-Authors Anni Anggraeni Shabarni Gaffar Yenni Wahyuni Hartati Yusuf Rohmatullah