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All Journal Journal of Food and Pharmaceutical Science Journal of Food and Pharmaceutical Sciences
Sri Priyantini
Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Hypoxia-Conditioned MSC-Derived Exosomes Mitigate Imiquimod-Induced Psoriasiform Dermatitis by Enhancing FOXP3 Expression and Normalizing Keratin-10 Expression Siti Lia Nurhasanah; Sri Priyantini; Eko Setiawan
Journal of Food and Pharmaceutical Sciences Vol 14, No 5 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.28643

Abstract

This study evaluated whether hypoxia-conditioned mesenchymal stem cell–derived exosomes (EH-MSC) mitigate imiquimod (IMQ)-induced psoriasiform dermatitis by enhancing immune regulation and restoring epidermal differentiation. Male Wistar rats (n=32) were used: 30 rats were randomized into five experimental groups (n=6 per group)—healthy control (G1), IMQ + saline (G2), IMQ + methotrexate (G3), IMQ + EH-MSC 100 µL (G4), and IMQ + EH-MSC 200 µL (G5)—and two additional rats were used for model validation. Psoriasiform dermatitis was induced using topical IMQ 5% for 7 days, followed by intradermal treatments (EH-MSC on days 1 and 3 post-validation). EH-MSC exosomes were generated from hypoxia-primed MSC cultures and confirmed by CD63/CD9 markers. Cutaneous FOXP3 and keratin-10 (K10) were quantified by qRT-PCR at the terminal time point. IMQ produced typical psoriasiform plaques and histologic changes. FOXP3 differed significantly among groups (ANOVA p<0.001) and increased dose-dependently with EH-MSC, peaking in G5 (1.84±0.29 pg/mL) versus G2 (0.65±0.33 pg/mL). K10 also differed significantly (p<0.001) and shifted toward healthy levels with EH-MSC, most closely in G5 (1.35±0.71 pg/mL) versus G2 (3.86±0.67 pg/mL). These findings show that EH-MSC treatment was associated with increased FOXP3 expression, suggesting enhanced Treg-associated immunoregulatory activity, and normalization of K10 expression toward healthy control levels.
Hypoxia-Conditioned MSC-Derived Exosomes Mitigate Imiquimod-Induced Psoriasiform Dermatitis by Enhancing FOXP3 Expression and Normalizing Keratin-10 Expression Siti Lia Nurhasanah; Sri Priyantini; Eko Setiawan
Journal of Food and Pharmaceutical Sciences Vol 14, No 5 (2026): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.28643

Abstract

This study evaluated whether hypoxia-conditioned mesenchymal stem cell–derived exosomes (EH-MSC) mitigate imiquimod (IMQ)-induced psoriasiform dermatitis by enhancing immune regulation and restoring epidermal differentiation. Male Wistar rats (n=32) were used: 30 rats were randomized into five experimental groups (n=6 per group)—healthy control (G1), IMQ + saline (G2), IMQ + methotrexate (G3), IMQ + EH-MSC 100 µL (G4), and IMQ + EH-MSC 200 µL (G5)—and two additional rats were used for model validation. Psoriasiform dermatitis was induced using topical IMQ 5% for 7 days, followed by intradermal treatments (EH-MSC on days 1 and 3 post-validation). EH-MSC exosomes were generated from hypoxia-primed MSC cultures and confirmed by CD63/CD9 markers. Cutaneous FOXP3 and keratin-10 (K10) were quantified by qRT-PCR at the terminal time point. IMQ produced typical psoriasiform plaques and histologic changes. FOXP3 differed significantly among groups (ANOVA p<0.001) and increased dose-dependently with EH-MSC, peaking in G5 (1.84±0.29 pg/mL) versus G2 (0.65±0.33 pg/mL). K10 also differed significantly (p<0.001) and shifted toward healthy levels with EH-MSC, most closely in G5 (1.35±0.71 pg/mL) versus G2 (3.86±0.67 pg/mL). These findings show that EH-MSC treatment was associated with increased FOXP3 expression, suggesting enhanced Treg-associated immunoregulatory activity, and normalization of K10 expression toward healthy control levels.
Co-Authors Eko Setiawan Siti Lia Nurhasanah