Background: Cervical cancer represents a critical global public health concern. According to the latest data from the WHO (Globocan 2024), cervical cancer ranks fourth among the most frequently diagnosed cancers in women worldwide, with an estimated 660,000 new cases and 350,000 deaths annually. The vast majority of these deaths (approximately 90%) occur in low- and middle-income countries, including Indonesia, where access to screening and early treatment remains severely limited. Cervical cancer caused by Human Papillomavirus (HPV) types 16 and 18 is the second leading cause of cancer-related mortality among women in Indonesia. Despite the IVA Test being a crucial strategy in reducing morbidity and mortality rates, national screening coverage in 2023 reached only 7.02% of the 70% target. Objective: This study aimed to analyze the association between knowledge level, attitude, and spousal support among Women of Reproductive Age (WRA) and their low participation in IVA Test screening at Clinic X in 2025. Methods: This study employed a observational analytic study with a cross-sectional design. The study population comprised all women of reproductive age between 15–50 years, with a sample of 88 respondents selected through simple random sampling. Results: The findings revealed that respondents' attitudes had a significant relationship on IVA Test screening participation (p = 0.01 < α = 0.05). In contrast, neither knowledge level (p = 0.982 > α = 0.05) nor spousal support (p = 0.152 > α = 0.05) demonstrated a statistically significant effect on IVA Test participation. Conclusion: Among the variables examined, the attitude of women of reproductive age emerged as relationship the low coverage of IVA Test screening. Knowledge level and spousal support did not yield statistically significant impacts in this study.