Improving teachers’ literacy and skills in utilizing microbes as plant growth promoters, as well as their understanding of eco-enzymes, are key factors in supporting sustainable agricultural practices at the Mataram State Vocational School of Agriculture (SMK PP Negeri Mataram). Furthermore, agricultural education remains dominated by conventional approaches based on simple organic fertilizers, such as manure, without optimizing the role of microorganisms. This community service activity aims to improve teachers’ literacy and skills in utilizing potential local bacterial isolates as bioinoculants in eco-enzyme formulations. The implementation method is participatory, involving the following stages: pre-test, outreach, training, independent practice, technology application, mentoring, and post-test. The innovation offered consists of a bacterial starter based on Plant Growth-Promoting Rhizobacteria (PGPR) isolated from the coastal region of Lombok Island, which is resistant to salinity and drought stress and has the potential to enhance plant growth. Pre-test results indicated that teachers’ initial understanding was still relatively low, particularly regarding potential microorganisms. Following the program, post-test results showed a significant improvement across all aspects, with scores ranging from 74 to 88, with the greatest improvement observed in the understanding of potential microbes. This activity also succeeded in shifting teachers’ paradigms from conventional organic farming practices toward a more scientific and practical microbiology-based approach. Thus, this program is effective in enhancing teachers’ capacity and has the potential to support the sustainable implementation of simple biotechnology-based learning in schools.