Adolescent girls are more vulnerable to iron deficiency anemia. Female adolescents have a higher risk of anemia, partly due to blood and iron loss through menstruation. According to WHO data, the prevalence of anemia among women aged 15–49 years was 29.9% in 2019 (WHO). In East Java Province, the prevalence of anemia among adolescents was 19.6% (East Java Health Office, 2020). The prevalence of anemia among adolescents in Jember Regency is relatively high, reaching 41% of the 76,000 adolescents (Jember District Health Office, 2023). To determine the change in hemoglobin levels after the administration of iron supplement tablets to adolescent girls in the Healthy School Movement Program at SMAN 5 Jember. This study used a pre-experimental design with a One-Group Pretest-Posttest Design. The sampling technique used was Proportionate Stratified Random Sampling, involving 30 adolescent girls. Data were analyzed using the paired sample t-test. The study showed that before taking the iron supplement tablets, the average hemoglobin level was 13.257 g/dL. After taking the supplements, the average hemoglobin level increased to 13.837 g/dL, with a difference of 0.58 g/dL indicating an improvement. The paired sample t-test showed a p-value of 0.017, which is less than α = 0.05, indicating a significant difference in hemoglobin levels. Therefore, the alternative hypothesis (H₁) was accepted. The administration of iron supplement tablets to adolescent girls for one month, at a dosage of one tablet once weekly and one tablet daily during menstruation, resulted in a change in hemoglobin levels — from a mean of 13.257 g/dL before treatment to 13.837 g/dL after treatment, with an increase of 0.58 g/dL. Respondents are advised to continue consuming iron supplement tablets regularly, at least once a week, in accordance with the recommendations from the school or health center. In this study sample, hemoglobin levels increased after one month of iron supplementation.