<![CDATA[Pujiastuti, Y., & Algifani, F. (2025). Utilization of Cattle and Rabbit Urine as Propagation Medium of Bacillus Thuringiensis and its Toxicity to Oryctes Rhinoceros in Laboratory. In: Herlinda et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-13 Tahun 2025, Palembang 20 Oktober 2025. (pp. x–y). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Biological control of insect pests using entomopathogens is gaining significant attention. One example is controlling of oil palm’s main pest Oryctes rhinoceros. The use of entomopathogen Bacillus thuringiensis is expected to control this pest. B. thuringiensis bacteria can be propagated in various growth media. The purpose of this study was to examine the growth of B. thuringiensis in various growth media and its effect on the mortality of O. rhinoceros. The study was conducted using a randomized block design, with five treatments and five replications. The five treatments were P1 (rice washing water + cow biourine), P2 (old coconut water + cow biourine), P3 (rice washing water + rabbit biourine), P4 (old coconut water + rabbit biourine). When mortality test was conducted, it was used P0 (water) as a control. The results showed different liquid waste formulations affected multiplication of B.thuringiensis after 72 hours. The mixture of rice washing water and rabbit biourine (P3) showed the highest results in producing spores, namely 11.69 x 109 spores/ml. The highest mortality rate was found in rice washing water and rabbit biourine mixture treatment (P3), and the lowest in the control treatment. The highest body length was obtained in the control treatment, which was 9.89 cm, and the lowest one was obtained in treatment P4 (old coconut water + rabbit biourine), which was 4.69 cm. The lowest LT50 value was 29.51 days and the lowest LT95 value was 65.30 days, shown by treatment P3 (rice washing water + rabbit biourine).]]>
