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All Journal Indonesian Journal of Jamu
Tabinna Riannissa
Universitas Ahmad Dahlan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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The Effect of Extraction Methods of Cassia alata L. Leaves on Flavonoid, Phenolic Contents, and Antifungal Activity Tabinna Riannissa; Sri Mulyaningsih; Hari Susanti
Jurnal Jamu Indonesia Vol. 11 No. 6 (2026): November–December 2026
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v11i6.464

Abstract

Cassia alata L. is used traditionally to treat fungal diseases such as tinea pedis (athlete's foot), caused by Trichophyton rubrum. This study examined how three extraction methods (maceration, reflux, and ultrasonication) applied to C. alata leaves affect total flavonoid content (TFC), total phenolic content (TPC), and antifungal activity. TFC and TPC were determined by UV–Vis spectrophotometry, and antifungal activity was assessed by the well diffusion method. Ultrasonication produced the highest TFC (4.68 mg QE/g), TPC (8.92 mg GAE/g), and antifungal activity (inhibition zone diameter 25.7 mm) compared with maceration and reflux. Ultrasonication was therefore more effective than maceration and reflux at extracting the constituents of C. alata leaves that act against T. rubrum.
Co-Authors Hari Susanti Sri Mulyaningsih