Background: Adolescence is a transitional period characterized by rapid physical growth and development, requiring adequate nutritional intake, particularly iron (Fe), which plays an essential role in hemoglobin formation. Iron deficiency can lead to anemia, a common nutritional problem among adolescent girls. The prevalence of anemia among adolescents in Aceh reached 33.8%, while in West Aceh Regency, it was recorded at 35.1% in 2023. Limited knowledge and inadequate access to health information contribute to low awareness and compliance with Fe tablet consumption. Health education through leaflet media is considered an effective approach because it provides concise, attractive, and easily accessible information that adolescents can read independently. Objective: This study aimed to determine the effect of health education using leaflet media on improving adolescent girls' knowledge regarding Fe tablet consumption at SMP Negeri 1 Meulaboh, West Aceh Regency. Methods: This study employed a quasi-experimental design using a one-group pretest-posttest approach. A total of 68 adolescent girls were selected as respondents through proportionate stratified random sampling. The study was conducted in September 2025. Data were collected using questionnaires administered before and after the health education intervention. The Wilcoxon signed-rank test was used to analyze differences in knowledge scores. Results: The statistical analysis revealed a significant difference in adolescent girls' knowledge before and after the health education intervention using leaflet media, with a p-value of 0.000 (p < 0.05) and a Z-value of -7.028. These findings indicate a significant improvement in knowledge following the intervention. Conclusion: Health education using leaflet media significantly improves adolescent girls' knowledge regarding Fe tablet consumption. Therefore, leaflets can be used as an educational medium to support anemia prevention programs among adolescent girls in schools.