Background: The increasing demand for broiler chicken meat requires increased production accompanied by the implementation of biosecurity measures to prevent the introduction and spread of diseases. Biosecurity encompasses conceptual, structural, and operational aspects that play an important role in maintaining animal health, improving productivity, and reducing economic losses. Objective: This study aimed to evaluate the level of biosecurity implementation and identify the constraints encountered in broiler chicken farming at PT. Ciomas Adisatwa Farm Tegineneng, Pesawaran. Methods: This study employed a descriptive method with a qualitative approach. Data were collected through observation, interviews, and documentation. The supporting instruments included a mobile phone and pen, while the research material consisted of a checklist-based observation sheet. The data were analyzed descriptively by calculating the percentage of biosecurity implementation based on the scores obtained. Results: The results showed that the overall level of biosecurity implementation reached 86.67%, which was categorized as very good. The operational aspect obtained the highest score of 91.66%, followed by the conceptual aspect at 88.89% and the structural aspect at 77.78%. Although all aspects were categorized as very good, the structural aspect still requires improvement in several supporting physical facilities. Conclusion: Overall, biosecurity implementation at PT. Ciomas Adisatwa Farm Tegineneng, Pesawaran, was very good through the implementation of conceptual, structural, and operational biosecurity measures. This implementation supports disease prevention and control, maintains animal health, and contributes to the sustainable productivity of broiler chicken farming.