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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioeksperimen: Jurnal Penelitian Biologi
Susanto, Febri Adi
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Agriculture, Biological Sciences & Forestry Education Environmental Science

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Optimasi Metode 1H-NMR Profiling pada Rimpang Kunyit (Curcuma domestica) Dwiseptianti, Caroline; Susanto, Febri Adi; Purwestri, Yekti Asih; Nuringtyas, Tri Rini
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9238

Abstract

The use of medicinal plants is increasing due to the lack of side effects caused and the number of bioactive compounds that cannot be represented by synthetic chemical synthesis compounds. However, the management and use of natural medicines for the main handling of diseases are often hampered by the quality of the ingredients which are low and unstable. The standardized quality control system of OAI (Indonesian Natural Medicine) is the main key to improve clinical assurance and safety of the use of herbal medicines in Indonesia. One of the medicinal plants known to the public is Curcuma longa L. (turmeric). The main active components contained in turmeric are curcumin, demetoksikurkumin, bis-demetoksikurkumin, and ar-turmeron. Information about the quality of turmeric is needed in its use as a raw material for drugs so we need an analytical technique that is able to identify the diversity of metabolite profiles of active compounds. In this research, an optimization method is used to improve efficiency in the extraction of turmeric rhizome metabolites so that the best solvent concentration is known for the analysis of fingerprinting secondary metabolites with 1H-NMR 500 MHz spectroscopy in turmeric rhizomes. The results were analyzed with MNOVA software and chemical shift obtained compared with the reference. From the results obtained a concentration of methanol-d4 (CD3OD) 100% able to extract curcumin better than other solvents. The solvent is able to extract saccharide (sugar) compounds in the form of sucrose, amino acids and fatty acids in the form of methionine, glutamine, acetate, and glycero phospho choline.
Pollen Morphology and Sucrose Giving on Stigma Relation with Incompability of Sweetpotato (Ipomoea batatas (L.) L.) Susanto, Febri Adi; Sulasmi, Eko Sri; Rahayuningsih, St A
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The purpose of the research is to examine how to increase cross and self-pollination by solving incompatibility of sweet potato mechanism through sucrose giving on stigma and pollen nuclei checking. Sucrose in different concentration is giving to stigma. Stigma is excised and lubricated with sucrose before crossing and the pedicel is lubricated with 2,4D 100 ppm in lanolin. The result shows both varieties have stigma position longer than the anthers do. Papua Solosa has 94.7% anther dehiscence, 70.25% pollen viability, while MSU 03028-10 has 87.3% anther dehiscence and 87.84% pollen viability. Both of these varieties have receptive stigma. Sucrose level in compatible pollination is lower than in incompatible pollination. Sugar in 50 ppm concentrate can increase fruit setting in self-pollination of Papua Solosa variety from 0% to 70%, 0% to 60% in MSU 03028-10, and 10% to 90% in crossing of Papua Solosa>Keywords : Pollen Morphology, Sucrose Giving, Incompatibility of Sweet Potato
Co-Authors Dwiseptianti, Caroline Eko Sri Sulasmi Rahayuningsih, St A Tri Rini Nuringtyas Yekti Asih Purwestri