Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal BERITA BIOLOGI
M, Hendro Santoso
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
KAJIAN PENGARUH SEL IMOBIL Arthrobacter NRRL B-3728 TERHADAP AKTIVITAS DAN STABILITAS ENZIM GLUKOSA ISOMERASE Triantarti, Triantarti; M, Hendro Santoso
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.062 KB) | DOI: 10.14203/beritabiologi.v6i3.1224

Abstract

This project focused on the immobilization of glucose isomerase (GI) from Arthrobacter B-3728. The whole cells immobilization technique using glutaraldehyde and gelatine type B220 was used in this research. The optimum time for harvesting Arthrobacter cells was determined before immobilization.The Arthrobacter cells were harvested after 56-72 h fermentation when the GI activity reaching 0.515-0.603 U/ml broth. The best treatment for cell immobilization was found using 5% gelatine when the GI activity reaching 0,888 U/ g immobilized cells. The optimum pH was not changed (pH 8) but the sensitivity to the pH was changed for immobilized cells compared to the free cells. However, the stability of the immobilized cells was lower compared to the free cells for long isomerization process. Further research are still needed for the development of immobilization technique for Arthrobacter B-3728.
Co-Authors Triantarti Triantarti