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Hulu, Adminarwati
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Agriculture, Biological Sciences & Forestry

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PROSPEK INTRODUKSI REGULON NIF DARI BAKTERI KLEBSIELLA PNEUMONIAE KE DALAM GENOM MITOKONDRIA TANAMAN Pranata, Ivan Tjahja; Hulu, Adminarwati; Handoko, Yoga Aji
Jurnal Penelitian Pertanian Terapan Vol 18, No 3 (2018)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v18i3.1509

Abstract

Use of synthetic fertilizers bring environmental pollution, increase weed growth, reduceatmospheric oxygen levels, decrease the fertility of agricultural land, causing eutrophicationand run-offs, also even damaging coral reefs. Recent biotechnology approaches can beutilized to create a new variety of self-fertilization plants that allows it to supply its ownnitrogen making it possible to overcome nitrogen problems and avoid them. This reviewaims to understand how far genetic engineering approach can be used by the introduction ofnif regulon from Klebsiella penumoniae. The nitrogenase-encoding genes which make up thenif regulon proved can be inserted into the mitochondrial genome as like Allen et al. (2017)did in their research. The western blotting result showed that the research succesfullyintegrated the nif regulon to the genome and expressed their proteins.
Co-Authors Pranata, Ivan Tjahja Purwoko, Agus Yoga Aji Handoko