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All Journal Jurnal Informasi Kesehatan Indonesia (JIKI) Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan) ARTERI : Jurnal Ilmu Kesehatan
sari, asri iman
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AKTIVITAS ANTIOKSIDAN KITOSAN PADA OVARIUM TIKUS BETINA YANG TERPAPAR TIMBAL (Pb) ASETAT sari, asri iman
Jurnal Informasi Kesehatan Indonesia (JIKI) Vol 4 No 2 (2018): Jurnal Informasi Kesehatan Indonesia
Publisher : Politeknik Kesehatan Kemenkes Malang (State Health Polytechnic of Malang)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.329 KB) | DOI: 10.31290/jiki.v(4)i(2)y(2018).page:80-86

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh kitosan terhadap ekspresi Bax dan jumlah atresia folikel pada ovarium tikus betina yang terpapar timbal asetat. Penentuan sampel dilakukan secara random, 25 ekor tikus betina sesuai kriteria inklusi dan ekslusi dibagi menjadi 5 kelompok. Semua kelompok, kecuali kelompok kontrol negatif diberi timbal asetat 175 mg/kg BB. Kelompok perlakuan 1, 2, dan 3 diberi kitosan yang dilarutkan dalam asam asetat 2% dengan dosis 16, 32, dan 64 mg/kg BB. Pemberian timbal asetat dan kitosan melalui sonde per oral. Aklimatisasai dilakukan selama 7 hari dan perlakuan selama 30 hari. Hari ke 31 dilakukan swab vagina. Jika tikus pada fase proestrus maka tikus dibedah dan diambil ovariumnya. Pengukuran ekspresi Bax menggunakan metode imunohistokimia dan penghitungan jumlah folikel atresia menggunakan metode hematoksilin eosin yang dilihat dengan mikroskop Olympus pembesaran 400x. Hasil penelitian menunjukkan bahwa kelompok perlakuan yang diberi timbal asetat dan kitosan berbagai dosis memiliki ekspresi Bax dan jumlah atresia folikel yang berbeda signifikan dengan kelompok kontrol negatif. Kata kunci: kitosan, antioksidan, timbal asetat ABSTRACT This research aims the effect of chitosan on bax expression and the number of follicle atresia in the ovary of female rat exposed lead acetate. Twenty five rats were divided into 5 treatment groups. All groups, except negative control group, were administered by 175 mg/kg BW oral lead acetate. Treatment group 1, 2, and 3 were treated using chitosan in dose of 16, 32, and 64 mg/kg BW dissolved in 2% acetic acid, respectively in 30 days. Before treated rat need acclimatization in 7 days. Administration of lead acetate and chitosan via sonde orally. Vaginal swabs in day 31, if rat in proestrus phase dissected and taken the ovary. Bax expression calculation with immunohistochemistry method and the number of follicle atresia calculation with hematoksilin eosin method. And then viewed use Olympus microscope zoom in 400. Results showed that Chitosan-treated groups in bax expression ang the number of follicle atresia differ significantly with negative control group. Keywords: chitosan, antioxidant, lead acetate
UPAYA PENINGKATAN STATUS GIZI PADA BALITA STUNTING DENGAN PEMERIKSAAN HB DAN MIKROBIOTA SEBAGAI UPAYA IMPLEMENTASI GERAKAN ANTI STUNTING Yuningsih, Yuningsih; Sari, Asri Iman; Zannah, Ai Nur
Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan) Vol. 8 No. 1 (2024): Jurnal IDAMAN (Induk Pemberdayaan Masyarakat Pedesaan)
Publisher : Politeknik Kesehatan Kemenkes malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31290/j.idaman.v8i1.4091

Abstract

Stunting, a type of malnutrition that begins in the womb until the first two years of life and is worsened by recurrent infectious diseases after birth. Children in tropical countries experience more worm infections which can cause blood loss through the digestive tract due to the number of bad microbiota in the digestive tract. Cumulatively, bad microbiota can cause malnutrition and blood loss which results in decreased body resistance and causes problems with children's growth and development. The aim of this community service is to examine the presence of gastrointestinal microbiota and HB which is associated with nutritional status in stunted toddlers. The method/stage of the activity is a screening examination starting with anamnese and physical examination, then Hb and feces examinations are carried out on stunted toddlers in the Kaliwates Health Center area, Kebonagung village. The number of stunted toddlers targeted was 17 aged 24 – 60 months out of 30 children whose parents were willing to have their Hb and feces checked. The result of this community service is that the number of bad microbiota is greater than the good microbiota in stunted toddlers and in this condition the Hb levels also decrease. Conclusion: Shifts in the composition of toddlers' intestinal bacteria have been associated with an increase in the number of gastrointestinal infections which resulted in a decrease in Hb levels in stunted toddlers.
Hubungan Perkembangan Balita Dengan Perilaku Dan Emosional Di Wilayah Puskesmas Kaliwates Jember Sari, Asri Iman; Zannah, Ai Nur; Yuningsih, Yuningsih
ARTERI : Jurnal Ilmu Kesehatan Vol 5 No 2 (2024): Februari
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v5i2.281

Abstract

The Indonesian Ministry of Health stated that around 400,000 toddlers in Indonesia suffer from developmental disorders. Based on Riskesdas 2013 data, the prevalence of stunting is 37.2%. Development can be assessed using motor skills, social and emotional skills, language skills, and cognitive skills. Children have the right to receive attention in the process of growth and development so as to achieve a good quality of life, to achieve a good future for the nation. The aim of this study was to analyze the relationship between the developmental status of stunting toddlers and their behavior and emotions at the Kaliwates Health Center, Jember district. The population of this study were 70 toddlers who came to posyandu in the Kaliwates Health Center, Jember Regency, and the sample was taken by total sampling. Measurement of growth and development of toddlers using the book Stimulation, Detection and Early Intervention of Growth and Development (SDIDTK). The research showed that 39 toddlers developed according to their age and 31 toddlers experienced developmental delays and required follow-up. Behavioral and emotional toddlers showed that 3 toddlers did not experience behavioral and emotional problems, while 67 toddlers experienced problems. The statistical test used the contingency coefficient and obtained a p-value of 0.047 with a significant level of 5%. It can be concluded that there is a relationship between the development of stunting toddlers with their behavior and emotions.
Hubungan Status Sosial Ekonomi dengan Kejadian Stunting pada Balita Usia 12-60 Bulan Di Puskesmas Kaliwates Yuningsih, Yuningsih; Sari, Asri Iman; Handayani, Yuni
ARTERI : Jurnal Ilmu Kesehatan Vol 4 No 4 (2023): Agustus
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v4i4.288

Abstract

Stunting is a condition of impaired growth and development in children that can result in morbidity, mortality, and motor development impairment. The occurrence of stunting is often associated with malnutrition. Jember is a regency with the highest prevalence of stunting in East Java in 2020. Socioeconomic status, such as parental education level and occupation, can influence access to adequate nutrition and healthcare, which in turn contribute to the risk of stunting. The objective of this study was to determine the relationship between socioeconomic status and the occurrence of stunting among children aged 12-60 months in Kaliwates Community Health Center. This study employed a quantitative approach with an analytical observational design and a cross-sectional study design. The study population consisted of 580 children. The sampling technique used in this study was Proportionate Stratified Random Sampling, calculated using the Issac and Michael formula, resulting in a sample of 58 respondents who met the inclusion criteria. The data were analyzed using univariate and bivariate analyses with statistical tests using Chi-Square. The Chi-Square test results indicated a significant relationship between fathers' occupation (p = 0.003), mothers' occupation (p = 0.000), fathers' education (p = 0.001), mothers' education (p = 0.000), and the occurrence of stunting among children aged 12-60 months in Kaliwates Community Health Center. To prevent and address the occurrence of stunting among children in Kaliwates Community Health Center, it is important to provide balanced nutrition education, improve access to nutritious food, regularly monitor children's growth and development, involve the community, strengthen parental roles, and conduct regular program monitoring and evaluation.
Hubungan Mikrobiota Saluran Cerna dengan Kadar Hb dan Status Gizi Anak Di Puskesmas Kaliwates Yuningsih, Yuningsih; Zannah, Ai Nur; Sari, Asri Iman; Handayani, Ririn
ARTERI : Jurnal Ilmu Kesehatan Vol 5 No 3 (2024): Mei
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v5i3.376

Abstract

The issue of nutritional status, particularly stunting in children, is a global concern that requires serious attention. This research aims to investigate the relationship between gut microbiota and hemoglobin (HB) levels and nutritional status in children at the Kaliwates Health Center. This type of research includes observational analytics with a cross-sectional study design. Sampling was done by purposive sampling, resulting in 19 respondents who met the inclusion criteria. The analysis of this study includes univariate and bivariate analysis with statistical tests using Chi Square. The research instruments used were digital Hb tests and laboratory feces examinations. The results of the univariate analysis using the Chi Square test showed that the majority of children's gut microbiota was abnormal (89.5%), while the majority of HB levels were normal (42.1%) and the majority of nutritional status was in the short category (47.4%). The bivariate analysis results of the relationship between microbiota and Hb levels showed a p-value of 0.215 (p-value>0.05), while the bivariate analysis results of the relationship between microbiota and nutritional status showed a p-value of 0.000 (p-value<0.05). The conclusion of this study is that there is no relationship between gut microbiota and Hb levels, but there is a significant relationship between gut microbiota and nutritional status.children's nutritional status, including the role of the microbiota in digestion and absorption of nutrients.
Co-Authors Ai Nur Zannah Handayani, Yuni Ririn Handayani Yuningsih Yuningsih