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Ruyitno, -
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Earth & Planetary Sciences

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ISOLATION AND DISTRIBUTION OF CRUDE OIL AND POLYCYCLIC AROMATIC HYDROCARBON-DEGRADING BACTERIA FROM POLLUTED HARBOURS IN NORTH JAKARTA Alfiansah, Yustian Rovi; Adindasari, Mindi; Argarini, Mentari; Darmayati, Yeti; Ruyitno, -
Marine Research in Indonesia Vol 39 No 2 (2014)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1616.668 KB) | DOI: 10.14203/mri.v39i2.49

Abstract

Several harbours in North Jakarta have been polluted by spills of oil and their derivates. We suggest that diverse species of crude oil and polycyclic aromatic hydrocarbon-degrading bacteria  inhabit these harbours. An experiment was undertaken in 2007 to isolate crude oil and polycyclic aromatic hydrocarbon (PAH)-degrading bacteria from oil-polluted harbours, such as Muara Baru, Sunda Kelapa and Tanjung Priok. Sea water and sediment samples were collected twice, in March and April. Crude oil and PAH-degrading bacteria were isolated from enrichment culture of samples in an enrichment medium (SWP), using ONR7a medium with the addition of 5 types of PAH gases or Arabian Light Crude Oil 210 (ALCO 210) onto medium. This study reported that fluoranthene and crude oil-degrading bacteria were the major bacteria isolated from the three polluted harbours. In total, 109 isolates have been collected which can degrade crude oil (29% of total isolates), fluoranthene (33%), fluorene (20%), pyrene (7%), dibenzothiopene (6%), and phenantrene (5 %). Among these isolates, 5 isolates have the capability to degrade 5 types of PAH and ALCO 210. They were Alcanivorax sp. B-1084, Pseudomonas sp. D5-38b, Alcanivorax sp. TE-9, Bacillus sp. L41, Alcanivorax dieselolei strain B-5 clone 1. Culturable bacteria have been isolated mostly from the Sunda Kelapa samples, with fewer in those from Muara Baru and Tanjung Priok, respectively
WATER QUALITY INDICATOR BACTERIA IN BENGAWAN SOLO AND PORONG RIVERS AND THEIR ESTUARIES Thayib, Soeminarti S.; Ruyitno, -; Kunarso, Djoko Hadi; Razak, Hamidah
Marine Research in Indonesia Vol 27 (1989)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1775.569 KB) | DOI: 10.14203/mri.v27i0.408

Abstract

Water samples taken from rivers and estuaries stations in Bengawan Solo and Porong rivers in East Java were analyzed for the pollution of indicator bacteria in July-August and NovemberDecember 1984, to determine the influences of domestic wastes on the quality of these waters. Higher frequency of occurrences of high total coliform, fecal coli and fecal streptococci were observed in water samples collected in river stations, river mouths and in the vicinity of dense populated areas. The distribution of saprophytes, freshwater bacteria and indicator bacteria were also studied. The changes in the bacterial population as funciton of the salinity were shown during the observations. Some pathogenic bacteria like Salmonella, Yarsinia, and Vibrio parahaemolyticus and others have been isolated from waste and mud samples.
DIRECT BACTERIA COUNTING IN THE BANDA SEA Ruyitno, -
Marine Research in Indonesia Vol 27 (1989)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1469.822 KB) | DOI: 10.14203/mri.v27i0.409

Abstract

Bacteria counting in the Banda sea were carried out in two seasons during Snellius II Expedition.  In August 1984, representing the southeast monsoon where upwelling is expected, bacteria counts were higher than in February 1985, representing the northwest monsoon where downwelling is expected. Some biological parameters of the Banda Sea were in support of the bacteria numbers in the expected upwelling region.
Co-Authors Adindasari, Mindi Alfiansah, Yustian Rovi Argarini, Mentari Djoko Hadi Kunarso Razak, Hamidah Thayib, Soeminarti S. Yeti Darmayati