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All Journal Journal of Stem Cell Research and Tissue Engineering Eduvest - Journal of Universal Studies
setyowardoyo, nugroho
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Aerospace Engineering Computer Science & IT Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Social Sciences Veterinary

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Analysis of The Effect of Service Income and Work Stress on Doctors' Organizational Commitment and Job Satisfaction as an Intervening Variable at dr. H. Jusuf SK of North Kalimantan Province Setyowardoyo, Nugroho; Utomo, Kabul Wahyu; Hidayat, Nurul
Eduvest - Journal of Universal Studies Vol. 5 No. 7 (2025): Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v5i7.50385

Abstract

This study aims to analyze the obstacles to the implementation of the JKN program related to patient admission, medical data processing, reporting, and funding at RSU Murni Teguh Medan. The formulation of the identified problems includes obstacles in the admission of BPJS Kesehatan patients, both outpatient and inpatient, medical data processing, reporting, and funding of BPJS patients. The purpose of the research specifically includes the analysis of the BPJS patient admission process and the obstacles faced, medical data processing, internal and external reporting, as well as the BPJS patient funding process, including the evaluation of obstacles based on the 6M elements (man, materials, machines, methods, markets, money). This research is expected to provide benefits, both for researchers as scientific experience, for hospitals to establish efficient service development strategies to improve service quality and patient satisfaction, and for BPJS Kesehatan to improve service quality, adjust policies to the development of science, and optimize service digitalization.
INFLUENCE OF LOW OXYGEN CONDITION OF BONE MARROW MESENCHYMAL STEM CELL setyowardoyo, nugroho
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 1 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.06 KB) | DOI: 10.20473/jscrte.v2i1.9267

Abstract

The state of low oxygen levels known as hypoxia in humans is considered a dangerous condition is apparently a normal physiological condition and required by the stem cells as they are in the body. Mesencyimal Stem Cells (MSCs) require physiologically optimal conditions of low O2 tension of 1-3% in the bone marrow. The purpose of this study was to reveal the difference between in vitro culture of MSC in normoxia condition (20% O 2 concentration) with hypoxia condition (1% O2 content) especially in terms of viability, pluripotent properties, and MSC proliferation ability of the culture it produces. This research is an explorative laboratory research invitro on Bone Marrow Mesenchymal Stem Cells (BMSCs) culture using hypoxia condition. The study design used Randomized Control Group Post-Test Only Design. This research was conducted for 2 months. There was a significant difference in mean slow proliferation based on the number of Least-like CFU-Cs between the control group, treatment group 1 and treatment group 2, the mean percentage of the number of cells expressing the OCT4 coding gene on immunofluorosense examination between the control group, 1 and the treatment group 2 and the mean percentage of cell numbers expressing the OCT4 coding gene on the immunofluorosense examination between the control group, the treatment group 1 and the treatment group 2 showing p <0.01. There was a significant difference of percentage of non-absorbing color cell number of trypsin blue (viable cells) between control group, treatment group 1, and treatment group 2 showing p value <0.05. This suggests that the precondition of culture with normoxia provides an opportunity for cells to adapt and proliferate before being conditioned in hypoxic cultures. Cultures with hypoxic conditions and preconditions of normoxia are the best culture conditions because they produce cells that are capable of maintaining pluripotency properties while still having better proliferation and viability capability compared with direct hypoxia conditions.Keywords: Hypoxia, normoxia, bone marrow, mesenchymal stem cells.
Co-Authors Kabul Wahyu Utomo Nurul Hidayat