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All Journal Jurnal Sain Peternakan Indonesia Journal of the Indonesian Tropical Animal Agriculture
Prihandini, P. W.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Melanocortin-4 Receptor (MC4R) gene polymorphism and its effect on growth traits in Madura cattle Prihandini, P. W.; Sumadi, S.; Suparta, G.; Maharani, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.38-46

Abstract

Melanocortin-4 receptor (MC4R) gene has an important role in the regulation of feed intake and energy balance control. The objective of this study was to identify the single nucleotide polymorphisms (SNPs) of MC4R gene and their association with growth traits in Madura cattle. A total of 198 calves were used in this study.Forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify approximately 493 bp of MC4R gene. The results showed that two SNPs, g.1133C>G and g.1108C>T were identified by direct sequencing. The PCR-RFLP method was performed to genotype all individuals studied based on SNP g.1133C>G, and its SNP was significantly associated with shoulder height (SH) at yearling age (P<0.05). Animals with GG genotype had a higher SH (110.35±6.40cm) than those with CC (102.00±8.00 cm) and CG genotype (105.96±6.23 cm). The SNP g.1133 C>G changed amino acid from valine to leucine. In conclusion, the SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.
Seleksi Calon Pejantan dan Induk Sapi Madura Berdasarkan Nilai Pemuliaan Berat Lahir dan Sapih Tribudi, Y. A.; Prihandini, P. W.; Rahaddiansyah, M. I.; Anitasari, S.
Jurnal Sain Peternakan Indonesia Vol 16 No 1 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.1.1-7

Abstract

Penelitian bertujuan untuk seleksi calon pejantan dan induk sapi Madura berdasarkan nilai pemuliaan berat lahir dan sapih di UPT Pembibitan Ternak dan Kesehatan Hewan Sapi Madura Pemekasan. Materi yang digunakan terdiri dari catatan produksi dan populasi sapi Madura tahun 2016 sampai 2019. Jumlah sapi Madura yang digunakan untuk mengestimasi nilai pemuliaan berat lahir dan sapih adalah 61 ekor (18 ekor calon pejantan dan 43 ekor calon induk) yang selanjutnya dilakukan koreksi untuk estimasi heritabilitas berdasarkan saudara tiri (paternal half sib correlation). Hasil penelitian menunjukkan nilai heritabilitas berat lahir dan berat sapih pada sapi Madura masing-masing adalah 0,41±0,37 dan 00,53±0,45. Rangking nilai pemuliaan calon pejantan sapi Madura berdasarkan berat lahir dan sapih tertinggi diperoleh pejantan no 20/911 (NP: 1,90 kg) dan 30/927 (NP: 16,06 kg) sedangkan pada calon induk diduduki oleh ternak no 10/680 (NP: 2,28 kg) dan 20/395 (NP: 16,48 kg). 
Co-Authors Anitasari, S. Maharani, D. Rahaddiansyah, M. I. S. Sumadi Suparta, G. Tribudi, Y. A.