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All Journal Majalah Ilmiah Peternakan Journal of the Indonesian Tropical Animal Agriculture
Dudi, D.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Leptin gene polymorphism of Ongole Grade cattle based on single nucleotide polymorphism Hilmia, N.; Rahmat, D.; Dudi, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.309-314

Abstract

Point mutation on exon 2 of leptin gene, which changes amino acid encoding from Arginine to Cysteine, may alters the physiological function of the leptin hormone. This study aimed to identify leptin gene polymorphism of Ongole Grade (OG) cattle based on Single Nucleotide Polymorphism (SNP). The DNA sample was taken from 48 head of OG cattle at Balai Pengembangan Perbibitan Ternak Sapi Potong(BPPT SP) Cijeungjing West Java, which was isolated from white blood cell using the high salt method. Amplification of DNA was done by Polymerase Chain Reaction (PCR), followed by direct sequencing to obtain nucleotide sequence. The SNP analysis was carried out from alignment of sequencing result using Bioedit and MEGA 5.2 program. The results indicated in exon 2 leptin gene of OG cattle there was one synonymous SNPs that did not changeamino acids Serine encoding on g.1025T >C/S17S, while two non synonymous SNPaltered amino acids encoding, those were g.1047C> T /R25C and g.1048G>A/R25H. Those mutations changed amino acids encoding from Arginine to Cysteine and Arginine to Histidine respectively.In OG cattle, the frequency of A allele (44.8%) was higher than C allele (33.3%) and T allele (21.9%). Six genotypes were also identified, i.e. AA (41.7%), CC (20.8%), CT (20.8%), CA(4.2%), TT (10.4%) and TA (2.1 %). Heterozigosity of OG cattle based on leptin gene was 0.65 that was a high category. The A allele was a specific allele on Indonesian local cattle.
PENINGKATAN KUALITAS PUPUK ORGANIK CAIR (POC) BERBAHAN DASAR URINE SAPI DENGAN PEMBERIAN MOLASE DAN EM4: SUATU KAJIAN PUSTAKA K. F., RIYANTO; DUDI, D.; HERNAMAN, I.
Majalah Ilmiah Peternakan Vol 27 No 1 (2024): Vol. 27 No. 1 (2024)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MIP.2024.V27.i01.p10

Abstract

The cattle industry in Indonesia is a growing livestock industry and this business has prospective opportunities. The increasing cattle industry in Indonesia will be in line with the waste produced. Ruminant livestock is one of the waste producers that has the potential to disrupt the environment if not managed properly. Utilization of cow feces and urine can be used as an organic fertilizer material that is environmentally friendly and can overcome waste problems. One of the uses of livestock waste is the manufacture of liquid organic fertilizer based on cow urine. Cow urine contains 95% water, 2.5% N and 2.5% other components (minerals, salts, hormones and enzymes). The composition of cow urine indicates that the nutrient content is good. However, the N-organic content of liquid organic fertilizer is very low if there is no improvement in the quality of liquid organic fertilizer. Improvement can be done by adding molase and EM-4 (effective microorganism-4) which contains organic matter that can improve the quality of the fertilizer produced. Liquid organic fertilizer from cow urine base material added with EM4 and molase can improve the quality of liquid fertilizer in terms of total N, C-organic, TDS and pH.
Co-Authors D. Rahmat I. Hernaman K. F., RIYANTO N. Hilmia