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All Journal Biogenesis: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology
Mahardhika, I Wayan Swarautama
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Phytochemical Properties, Antioxidant, and Cytotoxicity Activity of Knobweed (Hyptis capitata) from South Sulawesi, Indonesia To'bungan, Nelsiani; Widhiastuti, Stefani Santi; Hida, Fitriana Nur Laissya; Mahardhika, I Wayan Swarautama
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90976

Abstract

Hyptis capitata Jacq. known as Sumambu plants in Sulawesi, has phytopharmaceutical importance. H. capitata extracts were evaluated for their phytochemical properties, antioxidant activity, and cytotoxicity. Using the maceration yielded five types of extracts: root chloroform (RC), root methanol (RM), leaf chloroform (LC), leaf methanol (LM), and leaf ethanol (LE). Phytochemical properties were identified by qualifying procedure and digital image analysis for quantifying Red-Green-Blue (RGB) percentage and hex colour code. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to determine the half-maximal inhibitory concentration (IC50). Cytotoxicity screening of each extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against HeLa and 4T1 cells. Gas Chromatography-Mass Spectrometry (GC-MS) assay was used to identify the phytochemical compounds of the extracts with the most promising potential. Alkaloids were the major constituents of the phytochemicals of RC, RM, LE, LC, and LM. RM and LM have potency and weak free radical scavenging activities, with IC50 value 31.08 and 58.03 µg/mL, respectively. The IC50 of RC and RM against HeLa cells were 84.21 ± 0.63 and 172.10 ± 02.90 µg/mL, respectively. Meanwhile, the cytotoxicity of RC and RM against 4T1 cells were 86.42 ± 0.80 and 182.82 ± 7.00 µg/mL, respectively. It means RC and RM exhibit a moderate level of cytotoxicity in both HeLa and 4T1 cells. LM shows moderate cytotoxicity, but it is limited to 4T1 cells with an IC50 value of 181.86 ± 12.68 µg/mL. The cytotoxicity level of extracts was lower than doxorubicin. Campesterol, ferruginol, stigmasterol, cis-13-octadecenoic acid methyl esters, and methyl palmitate were predicted to play a role in the antioxidant activity and cytotoxicity of RC, RM, and LM. RC, RM, and LM possess the potential for development as anticancer agents. Moreover, RM shows promise as an antioxidant due to its notable radical scavenging activity. Further research is required to explore the cytotoxic effects of RC, RM, and LM on normal cells and to assess their toxicity in experimental animals. 
Inheritance and Comparison of Phenotypic Characters from Hybrid Chicken GK-Bro (Gallus gallus Linnaeus, 1758) Damayanti, Putu Ayu; Daryono, Budi Setiadi; Mahardhika, I Wayan Swarautama
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 2 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i2.9493

Abstract

GK-Bro (Gallus gallus Linnaeus, 1758) is a hybrid chicken from crossing between ♀ F2 Broiler ♂ F2 Golden Kamper that have variation of phenotypic character. To meet the market’s demand, GK-Bro must have prime phenotypic character in both quantity and quality. This research aims to examine the phenotype character that occur in GK-Bro by the character of feet morphometry, crest’s shape, feather’s color, and feet’s color. The parental chicken are crossed in Innovation and Agriculture Centre of Gadjah Mada University (Indonesia) and produce 11 chickens with five males and six females filial. The filial are maintained intensively and qualitative data were observed at the end of seven weeks, classified based on the percentage of the phenotypic class. Quantitative characters that observed were tibia and femur length after seven weeks, using control pelung, broiler, and F1 broiler. Quantitative data showed that the average length of the tibia and femur of GK-Bro is lower when compared to the control but the ratio of feet morphometry is closer to pelung. Qualitative data classified crest form in one class, feather’s color in 4 classes and feet’s color in 3 class. 
Morphological Traits, Productive Performance and Genotyping Fat Deposition PPAR Gene in Gama Ayam Crossbreeds of Female F1 Kamper and Male BC1 Kambro Ummah, Isna Mustafiatul; Mahardhika, I Wayan Swarautama; Daryono, Budi Setiadi
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 2 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i2.9993

Abstract

On the recent classification of native Indonesian chicken, there are 31 breeds of chicken. Highly circulated chicken breed in Indonesia is fast-growing broiler that includes several strains such as Cobb 500, Hubbard, Hybro, Isa Hyline, and Hisex. Fast-growing broiler chickens have rapid growth, with excessive fat deposition on chicken carcass which implicates a health problem and unfavorable meat quality. Gama Ayam Research Team conducted selective breeding which produces two new chicken breed Kamper and Kambro. Further selective breeding then crossbred female F1 Kamper and male BC1 (backcross I) Kambro. In this research morphological traits, productive performance and genotyping of PPAR gene related to fat deposition gene and blood lipid content in Gama Ayam were identified. Based on data analysis hybrid chickens morphological traits it concluded that Gama Ayam has a significant variation based on feather color. The productive performance was determined with the feed-conversion ratio (FCR) value which was 3.17. Genotyping of PPAR gene resulted in four polymorphisms that formed 14 haplotypes groups. Based on blood lipid content analysis of cholesterol content, triglycerides content, HDL and LDL, Gama Ayam have significantly lower content of cholesterol (107.05 mg/dl), LDL (44.71 mg/dl) and triglycerides (22.41 mg/dl). PPAR gene polymorphisms are not correlated with blood lipid content in Gama Ayam. A significantly strong correlation between PPAR gene polymorphism on the bodyweight of Gama Ayam at 49-days-old. Triglyceride level, cholesterol level and LDL level in Gama Ayam were lower than broiler chicken. Further research with a larger population size and sex classification of hybrid chicken must be conducted to validate the results.
Co-Authors BUDI SETIADI DARYONO Damayanti, Putu Ayu Hida, Fitriana Nur Laissya To'bungan, Nelsiani Ummah, Isna Mustafiatul Widhiastuti, Stefani Santi