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Inkiriwang, Annatje E.B.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Social Sciences

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Substitusi Media Murashige dan Skoog/MS dengan Air Kelapa dan Pupuk Daun Majemuk pada Pertumbuhan Anggrek Dendrobium secara in vitro (In Vitro Growth of Dendrobium Orchids under Substitution Murashige dan Skoog/MS Medium With Coconut Water and Compound Leaf Fertilizer) Inkiriwang, Annatje E.B.; Mandang, Jeany; Runtunuwu, Semuel
JURNAL BIOS LOGOS Vol 6, No 1 (2016): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.6.1.2016.16258

Abstract

Abstrak  Penelitian ini bertujuan  untuk  menentukan  konsentrasi  substitusi air kelapa dan pupuk   daun  majemuk  yang tepat    pada   media  MS   terhadap   pertumbuhan   anggrek Dendrobium secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 10 kali ulangan.  Variabel yang diamati adalah persentase eksplan yang bertunas, jumlah tunas, jumlah daun, tinggi tanaman dan jumlah akar. Hasil penelitian menunjukkan substitusi media MS 50 %, air kelapa 30 % dan pupuk daun majemuk (Growmore®) 1,5 g/L menghasilkan rata-rata persentase eksplan yang bertunas sebesar 6,78 %; jumlah tunas sebesar 1,26 dan tinggi tanaman sebesar 1,20 cm.Kata kunci: air kelapa, in vitro, media MS, pupuk daun majemuk  Abstract             This research was aimed to determine the proper concentration of substitution of coconut water and compound leaf fertilizer in Murashige dan Skoog (MS) medium on the in vitro growth of Dendrobium orchids. The completely randomized design experiment consisted of 7 treatments and 10 replicates. The measured parameters were the percentage of sprouted explants, number of shoots, number of leaves, plant height and number of roots. The result showed that the substitution of 50% media MS, 30% coconut water and 1.5 g/L leaf fertilizer (Growmore®) resulted the growth of shoot explant as much as 6.78% the shoot number of 1.26 and 1.20 cm plant height .Keywords: coconut water, compound leaf fertilizer, in vitro, MS medium 
Pengaruh Jenis Dan Konsentrasi Auksin Terhadap Pertumbuhan Dan Perkembangan Tanaman Anggrek Dendrobium mirbelianum Secara In Vitro Galensong, Justio P.D.; Tilaar, Wenny; Rogi, Johannes E.X.; Lengkong, Edy F.; Walingkas, Stanley A.F.; Inkiriwang, Annatje E.B.
AGRI-SOSIOEKONOMI Vol. 20 No. 2 (2024): Agri-Sosioekonomi
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/agrsosek.v20i2.57367

Abstract

This study aims to determine the effect of auxin type and concentration on the growth and development of orchid plants. The research was conducted at the Tissue Culture Biotechnology Laboratory, Faculty of Agriculture, Sam Ratulangi University Manado, and was carried out over 2 months from July to August 2023. This study used a factorial experiment in a Completely Randomized Design (CRD) with 5 replications consisting of two factors. The first factor is the type of auxin: IAA (A1), NAA (A2), and IBA (A3), and the second factor is the concentration of each auxin: 1 ml/l (K1), 2 ml/l (K2), and 3 ml/l (K3). The data were analyzed using analysis of variance, and if the results were significant, a 5% Least Significant Difference (LSD) test was conducted. The results showed that there was an interaction between the type and concentration of auxin on variables such as the number of roots, root length, root emergence time, number of leaves, number of shoots, and shoot height. Similarly, there was an effect of auxin concentration on each type of auxin, but there was a significant effect of individual auxin types (IAA, NAA, and IBA) on the root formation of Dendrobium mirbelianum orchids, affecting variables such as the number of roots, root length, root emergence time, number of leaves, number of shoots, and shoot height.
Co-Authors Edy F. Lengkong Galensong, Justio P.D. Jeany Mandang Johannes E. X. Rogi Runtunuwu, Semuel Stanley A.F. Walingkas Wenny Tilaar