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All Journal Bioma : Berkala Ilmiah Biologi The Journal of Experimental Life Sciences (JELS)
Fatma, Lania Yuchanitz
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Neuroscience

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Keanekaragaman Dioscorea spp dan habitatnya di Kabupaten Kudus, Jawa Tengah Diversity and habitat of Dioscorea spp in Kudus, Central Java Fatma, Lania Yuchanitz; Jumari, J; Utami, Sri
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.325 KB) | DOI: 10.14710/bioma.20.1.17-24

Abstract

Dioscorea spp (Yam) is one of the potential local food producers. It has advantage of high species diversity, containing high carbonhydrate and easy to grow. The aim of this research is to determine the variants of Dioscorea spp, examine the structure and composition of trees around Dioscorea spp habitat and determine the enviromental factors of Dioscorea spp habitat. The research was carried out in May-June 2017. The sampling technique was using exploration and quadratic method by plotting the trees into 5 squares (10 m x 10 m) with diameter of stem more than 10 cm. The name of each species, number of individuals and stem diameter measurement of each plot were recorded. The data of diversity Dioscorea spp and environmental factors were analyzed by sort of descruptive set and the trees was analyzed by vegetation analysis. The results showed that 4 species of Dioscorea spp. There are D. hispida, D. bulbifera, D. alata and D. esculenta. D. alata has 3 variants called uwi ireng, uwi ungu and uwi bangkulit. D. esculenta has 2 variants called gembili bebek and gembili terpong. The trees around Dioscorea spp obtained 21 species with the most important index is banana (Musa paradisiaca) and sengon (Albizia chinensis). The environmental factors were indicated the appropiate growth of Dioscorea spp. Key words: Dioscorea spp, local food stuffs and variant types
Resistance Test of Potential Mercury-Reducing Bacteria Using Agar Plate Method Amandita, Fitri Yola; Efadeswarni, Efadeswarni; Aini, Nabillah; Fatma, Lania Yuchanitz
The Journal of Experimental Life Science Vol. 14 No. 2 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.02.05

Abstract

The restoration of mercury-contaminated sites is a global concern, and countless studies have been conducted to formulate the best remediation practice. Mercury pollution in Indonesia has been a national issue. Thus, the government has enforced the reduction and elimination of this toxic and persistent chemical element and its compounds since 2019. As claimed to be the most environmentally friendly and cost-efficient technique, bioremediation using indigenous bacteria offers excellent potential to be implemented in developing countries such as Indonesia. This study is a preliminary part of four-year research at formulating a bioremediation technique to restore mercury-contaminated soils, especially in agricultural areas. In this investigation, fifteen indigenous bacterial isolates were tested for their resistance to mercury using two different techniques: the agar plate method, namely the disk-diffusion technique, and a modified version of the cup-plate technique. Inhibitory zone diameter (IZD) was measured and compared using Bland-Altman analysis between two techniques with supplementation of HgCl2 2-100 ppm range to the Mueller-Hinton agar media. The disk-diffusion technique appeared to give inconsistent measurements of IZD along the increase of HgCl2 concentration, in which the highest IZD mean was obtained from samples with 8 and 10 ppm HgCl2. Meanwhile, the cup-plate technique shows a lower average of IZD compared to the disk-diffusion technique. This report provides insights on the resistance of potential bacteria for mercury bioremediation. Further study should consider the accuracy of the agar plate method to test the bacterial resistance to any particular compound. Keywords: bioremediation, cup-plate, disk-diffusion, inhibitory zone diameter, method accuracy.
Co-Authors Aini, Nabillah Amandita, Fitri Yola Efadeswarni, Efadeswarni Jumari, J Sri Utami