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All Journal Bioma : Berkala Ilmiah Biologi Berkala Bioteknologi
Naufal, Adhitya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Identifikasi Jenis Pigmen Dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi Dari Sedimen Sumber Air Panas Gedong Songo Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.736 KB) | DOI: 10.14710/bioma.19.2.95-103

Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%. Keywords: Serratia marcescens, prodigiosin, KLT, antioxidant
Identifikasi Jenis Pigmen dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi dari Sedimen Sumber Air Panas Gedong Songo Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Berkala Bioteknologi Vol. 2 No. 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Pigmen dapat dihasilkan oleh tumbuhan, hewan dan mikrobia, termasuk bakteri Serratia marcescens. Pigmen diyakini dapat digunakan sebagai antibakteri, antikanker, antibiotik dan antioksidan. Kualitas pigmen sangat ditentukan oleh jenisnya. Tujuan penelitian ini adalah identifikasi jenis pigmen yang diproduksi oleh S. marcescens hasil isolasi sedimen sumber air panas Gedong Songo, dan uji aktivitas antioksidannya. S. marcescens ditumbuhkan pada media Nutrient Broth (NB) selama 96 jam pada temperatur ruang dan tiap 6 jam sekali dilakukan pengambilan sampel untuk pengukuran pertumbuhan dan pengukuran pigmennya. Pengukuran pertumbuhan S. marcescens menggunakan berat kering sel, sedangkan pengukuran produksi pigmennya dilakukan menggunakan spektrofotometer dengan panjang gelombang 535 nm untuk pigmen merah serta identifikasi pigmen dengan Kromatografi Lapis Tipis (KLT) dan UV- Vis dan pengujian aktivitas antioksidannya menggunakan metode carotene bleaching. Hasil menunjukkan pengukuran produksi pigmen memiliki titik optimal pada jam ke 48 sebesar 1,319 mg/L. Identifikasi jenis pigmen S. marcescens menggunakan KLT didapatkan nilai  0,8 dengan panjang gelombang pada spektrofotometer sebesar 536 nm. Nilai aktivitas antioksidannya PigmenS. marcescens didapatkan sebesar 13%.
Identifikasi Jenis Pigmen dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi dari Sedimen Sumber Air Panas Gedong Songo Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Berkala Bioteknologi Vol. 4, No. 1, April 2021
Publisher : Berkala Bioteknologi

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Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%.
Co-Authors Budi Raharjo Endang Kusdiyantini