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All Journal AL KAUNIYAH Jurnal Biologi Tropis PENDIPA Journal of Science Education
Aisyah, Arina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Mathematics

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OPTIMASI DAN PEMEKATAN LIPASE Bacillus halodurans CM1 Aisyah, Arina; Mangunwardoyo, Wibowo; Trismilah, Trismilah; Suhendar, Dadang
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.189 KB) | DOI: 10.15408/kauniyah.v10i2.4908

Abstract

Abstrak Lipase diketahui memiliki peranan penting dalam bidang industri. Produksi lipase dapat dihasilkan oleh kapang, khamir, dan bakteri. Penelitian bertujuan untuk meningkatkan aktivitas lipase yang dihasilkan oleh Bacillus halodurans CM1. Aktivitas lipase dapat ditingkatkan dengan optimasi komposisi media, mutasi bakteri dengan radiasi gamma dan N-methyl-N’-nitro-N-nitrosoguanidine (NTG). Enzim yang dihasilkan dipekatkan dengan metode stirred-cell ultrafiltration (UF)-ammonium sulfat dan UF-Polyethylene glycol (PEG). Uji aktivitas dilakukan pada tujuh media yang berbeda untuk mendapatkan media produksi. Delapan variabel komposisi media dioptimasi dengan rancangan Plackett-Burman. Bakteri dimutasi dengan radiasi gamma dosis 0,1–0,4 kGy dan NTG 0,05–0,15 mg/mL dengan waktu inkubasi 1–3 jam. Hasil penelitian menunjukkan bahwa media produksi yang digunakan berdasarkan optimasi media dan komposisi media Plackett-Burman adalah media dasar Bora & Bora yang mengandung 0,5% palm oil (PO) dan 0,09% CaCl2. Aktivitas lipase optimal diproduksi oleh bakteri hasil mutasi dengan NTG 0,1 mg/mL yang diinkubasi selama 3 jam. Pemekatan enzim UF-ammonium sulfat dan UF-PEG mampu meningkatkan aktivitas enzim lipase sebesar 18,44%.  Abstract Lipase is known to have an important role in the industrial field. Lipase can be produced by molds, yeasts, and bacteria. The research aimed to increase the activity of lipase produced by Bacillus halodurans CM1. Lipase activity can be improved by optimization of the composition of the media, the mutation of bacteria with gamma radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The enzyme was concentrated by stirred-cell ultrafiltration method (UF)-ammonium sulfate and UF-Polyethylene glycol (PEG). The activity test was performed on seven different media to get production media. The eight variables of the media composition were optimized by Plackett-Burman design. The bacteria were subject to mutation by using 0.1–0.4 kGy dose of gamma radiation and 0.05–0.15 mg/mL NTG with incubation time for 1–3 hours. The results showed that the production media used based on optimization and composition of Plackett-Burman media was Bora Bora medium that containing 0.5% palm oil (PO) and 0.09% CaCl2. Optimum lipase activity was produced by the bacterium that mutated with 0.1 mg/mL NTG, incubated for 3 hours. The concentrated by UF-ammonium sulfate and UF-PEG could increase the lipase activity by 18.44%.
Identifikasi Keanekaragaman dan Kelimpahan Zooplankton di Danau Sunter DKI Jakarta Chusna, Jhihan Hanifa; Aisyah, Arina; Afandi, Ahmad Yusuf
PendIPA Journal of Science Education Vol 8 No 2 (2024): June
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.8.2.330-336

Abstract

Zooplankton in the food chain acts as the primary consumer which connects phytoplankton as producers with organisms that have higher nutritional levels, for example nekton and benthos. This research aimed to identify zooplankton diversity and abundance in Lake Sunter. This research used a purposive sampling method to take the samples, to determine station points based on the environmental conditions of the lake. The results obtained were the identification of species from the genus Candacia, Nauplius, Eudiaptomus, Tintinnopsis, Polyarthra, Brachionus, Trichocerca, Philodina, dan Polydora. The diversity index of station 1 was categorized as medium (1.32), station 2 was in the low category (0.64), and station 3 was in the medium category (1.47). The abundance value at station 1 was 26,000 Ind/L, station 2 had an abundance value of 9,302 Ind/L, and station 3 had an abundance value of 32,500 Ind/L. In conclusion, the results of research at Lake Sunter found 9 genera of zooplankton. The diversity index of Lake Sunter was in the low to medium category, which indicates that the area has high ecological pressure and the species diversity index is low. The zooplankton abundance index value was categorized as high because each station had an abundance value above 500 Ind/ L.
Identification of Plankton Diversity and Abundance at Situ Gintung South Tangerang City Fatmala, Qori Diah; Aisyah, Arina; Afandi, Ahmad Yusuf
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7348

Abstract

Plankton are organisms that are found living floating in the water, that move passively so they are easily carried by currents. Plankton includes two large groups, namely phytoplankton which is similar to plants and zooplankton which similar to animals. One of important benefits of plankton is an indicator of biological conditions. This research aims to determine the types of plankton and their abundance at Situ Gintung, South Tangerang City. Sampling was conducted at three points area. The samples obtained were then observed and counted under a microscope and identified using web determination. Data were analyzed using plankton diversity and abundance formulas. The research results found phytoplankton species, such as Nitzschia sp., Navicula sp., Coscinodiscus sp., Synedra sp., Staurastrum sp., Scenedesmus sp., Coelastrum sp., Oscillatoria sp., Spirulina sp., Peridinium sp., and Phacus sp. The zooplankton obtained were Nauplius sp., Eudiaptomus sp., Polyarthra sp., Brachionus sp., Trichocerca sp., and Lecane sp. The average value of the phytoplankton diversity index is 0.543 and zooplankton is 1.043. The average phytoplankton abundance index obtained is 18,808,334 ind/L and zooplankton is 32,766 ind/L. Based on the results obtained, it was concluded that there were 11 types of phytoplankton and 6 types of zooplankton. The phytoplankton diversity index is in the low category and zooplankton is in the medium category, while the phytoplankton and zooplankton abundance index are in the medium to high category, but phytoplankton abundance is more dominant than zooplankton.
Co-Authors Afandi, Ahmad Yusuf Chusna, Jhihan Hanifa Fatmala, Qori Diah Suhendar, Dadang Trismilah Trismilah WIBOWO MANGUNWARDOYO