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Assessment of Genetic Diversity Using Morphological and Molecular Characteristics of Indonesian Zoysiagrass Genotypes Rahayu; Fatimah; Mo, Yang Geun; Bae, Eun Ji
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.263-277

Abstract

Zoysiagrass is warm-season turfgrass thriving in tropical regions. Despite the adaptive nature, the existence of Indonesian zoysiagrass as well as morphological and genetic characteristics are not available. Therefore, this study aimed to explore Indonesian zoysiagrass from western and eastern parts of the country, as well as its morphological and molecular characteristics. Morphological characteristics was conducted to measure vegetative and reproductive characters while genotyping was performed using 15 simple sequence repeat markers. Morphological characteristics cluster three major groups, namely Group 1 corresponded to short, shorter, and fine leaves. Group 2 corresponded to tall, longer, and fine leaves, fewer seeds, and short spikelet, while Group 3 corresponded to tall, long, and wider leaves, more seeds, and longer spikelet. The results showed that the expected heterozygosity (He = 0.256) was lower than homozygosity (Ho = 0.341). The high level of discriminating capacity, polymorphism, and informativeness of SSR marker was observed (Effective Multiplex Ratio = 4.20, Marker Index = 2.394, and Resolving Power = 1.574). Additionally, population structure generated two subpopulations. Group 1 corresponded to Z. japonica from Sumatera Island and mixed province while Group 2 corresponded to Z. japonica from Central Java and Bali with Z. japonica and Z. matrella from mixed province. In conclusion, the exploration of morphological and genetic diversity from Indonesian zoysiagrass provided useful insight for conservation and future breeding improvement.
Assessment of Land Quality for Siamese Orange (Citrus nobilis var. microcarpa) Development in Pacitan Regency, Indonesia Rahayu, Rahayu; Supriyadi, Supriyadi; Sumani, Sumani; Herawati, Aktavia; Dewi, Kurnia Mukti; Mo, Yang Geun; Bae, Eun Ji
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 5, No 1 (2024): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v5i1.80729

Abstract

Siamese oranges are one of the most widely developed horticultural crops in Indonesia. Arjosari and Ngadirojo are some of the sub-districts that produce Siamese orange in Pacitan Regency. One of the factors that can affect the development of citrus is land quality. This study aims to obtain land quality index data and improvement efforts for developing Siamese orange. Soil samples were taken from 25 land mapping units (LMUs) with 87 sample points. The soil sampling points were determined based on area or sampling density. Data analysis used for soil quality assessment includes correlation, principal component analysis (PCA), minimum data set (MDS), and calculation of climate quality index. The parameters used in this study are bulk density, porosity, moisture content, texture, pH, electrical conductivity (EC), cation exchange capacity (CEC), base saturation, total N, available P, available K, soil organic carbon (SOC), microbial biomass carbon (MBC), evapotranspiration, temperature, and solar radiation. Based on the results of soil quality analysis in the study area, it has a value range of 0.28 to 0.37, including low class, while the climate quality index is 1.39 with moderate class. The land quality index has a very low to low class with a value of 0.39 to 0.51. Intrinsic, extrinsic, and anthropogenic factors can influence soil quality. The study area’s limiting factors for developing Siamese oranges are EC, total N, and available P, which can be improved by making furrow ponds, adding organic fertilizer, using urea fertilizer, and SP36 according to the dosage.