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All Journal Acta Pharmaciae Indonesia : Acta Pharm Indo Makara Journal of Science
Suparto, Irma
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Medicine & Pharmacology

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PURIFIKASI DAN PENCIRIAN ENZIM PROTEASE FIBRINOLITIK DARI EKSTRAK JAMUR MERANG Sajuthi, Dondin; Suparto, Irma; Yanti, Yanti; Praira, Willy
Makara Journal of Science Vol. 14, No. 2
Publisher : UI Scholars Hub

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Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract. Edible straw mushroom (V. volvaceae) has been known used for improvement of blood circulation due to its fibrinolytic content. The objective of the study is to purify and characterize fibrinolytic protease from straw mushroom extract. Purification were performed through several steps, i.e. precipitation using ammonium sulphate 75%, dialyzed membran (cut-off 10 kDa), and ion-exchange chromatography using DEAE Sepharose. The active fraction of DEAE-Sepharose contains two purified protein bands with molecular weight of 12.9 and 15.8 kDa. The active fraction has specific activity of 0.383 U/mg with 2.7 fold higher purification compared to its crude extract. Both crude and purified enzymes had optimum activity at temperature of 50 ºC and pH 7 in 10 minutes of incubation. Fibrin zymographic profile demonstrated that the enzyme hydrolyzed fibrin, as well as casein, indicating their potent fibrinolytic activity. The enzyme was strongly inhibited by phenilmethylsulphonyl fluoride and N-p-tosil-L-lysinchloromethyl keton. This suggested that it was a serine protease. In summary, these results showed that crude and purified protease of straw mushroom (V. volvaceae) has fibrinolytic activities that can be applied for alternative thrombolytic therapy
Fraksi Alkaloid Daun Ubi Jalar Ungu (Ipomoea batatas var Ayumurasaki) Sebagai Inhibitor α-Glukosidase Pamungkas, Diah Daru; Batubara, Irmanida; Suparto, Irma
Acta Pharmaciae Indonesia Vol 4 No 2 (2016): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

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Ubi jalar ungu (Ipomoea batatas var Ayumurasaki) dilaporkan dapat membantu diet harian bagi penderita diabetes melitus tipe 2 karena memiliki kadar glikemik yang rendah. Salah satu mekanisme menurunkan kadar gula darah pada penderita diabetes adalah dengan menghambat enzim α-glukosidase. Potensi fraksi alkaloid dari daun dan fraksi antosianin dari umbi ubi jalar ungu dievaluasi sebagai inhibitor enzim α-glukosidase. Ubi jalar ungu yang digunakan dari Ciampea, Bogor, Jawa Barat. Fraksi alkaloid daun ubi jalar ungu pada konsentrasi 2,00% aktif menghambat kerja enzim sebesar 61,88%, sedangkan fraksi antosianin umbi ubi jalar ungu tidak aktif (0,25%). Fraksi alkaloid daun ubi jalar ungu dipisahkan lebih lanjut menggunakan kromatografi kolom dengan fase diam silika gel dan kloroformsebagai eluen. Fraksi yang mengandung alkaloid dengan semprotan Dragendorf, yakni F10, kemudian dipisahkan kembali dengan kromatografi lapis tipis preparatif. Fraksi F10.15 positif mengandung alkaloid dan dapat menghambat enzim -glukosidase sebesar 18,07% pada konsentrasi 1,25%, yang berarti pemisahan menyebabkan penurunan aktivitas. Spektrum inframerah transformasi Fourier fraksi memperlihatkan gugus fungsi –NH−, –OH, dan –CH2– dan diduga mengandung alkaloid.
Co-Authors Dondin Sajuthi Irmanida Batubara Pamungkas, Diah Daru Praira, Willy Yanti Yanti